NACL胁迫对不同基因型大麦花药离体培养的影响

EFFECTS OF NaCl STRESS ON ANTHER CULTURE FOR DIFFERENT GENOTYPES IN BARLEY

以2份耐盐大麦和3份未经耐盐性鉴定的大麦为供试材料,比较了它们的离体培养花药对诱导培养基中添加0.3%NACL、愈伤组织对分化培养基中添加0.3%NACL的培养反应。实验结果表明:在不添加NACL诱导培养基上培养的花药反应率与供试材料耐盐性无相关性;不添加NACL培养基上形成的愈伤组织在不添加NACL分化培养基上形成绿苗分化率与供试材料的耐盐性相关性不明显;添加NACL培养基上形成的花药反应率与供试材料耐盐性呈明显相关;添加NACL培养基上形成的绿苗分化率与供试材料的耐盐性相关性明显;愈伤组织诱导和苗分化二阶段连续给予NACL胁迫比分别给予NACL胁迫更易鉴定出供试材料的耐盐性。

Using 2 barley genotypes with higher salt-tolerance and 3 barley genotypes with undetermined salt-tolerance as donor materials, the culture response of in vitro culture of anthers to induction medium with 0.3 % NaCl and the culture response of callus to differential medium with 0.3 % NaCl were investegated. The results showed： 1 ） No relationship was existed between the frequency of anther responding formed on NaCl-free induction medium and the salt-tolerance of donor materials. 2） The relationship was not obviously existed between the frequency of green plant regeneration formed on the NaCl-free differential medium culturing calli inducted from NaCl-free induction medium and salt-tolerance of donor materials. 3 ） The relationship was obviously existed between the frequency of anther responding formed on the medium added with O. 3 % NaCl and salttolerance of donor materials. 4 ） The relationship was obviously existed between the frequency of green plant regeneration formed on the medium added with 0. 3 % NaCl and salt-tolerance of donor materials. 5 ） The materials with higher salttolerance was more easily selected from the procedure of making NaCl stress during the callus induction and regeneration stages compared to one stage.