鸡肉组织中二甲硝咪唑残留物测定

Detection of metabolites of DMZ in chicken muscle tissue by HPLC-ESIMS

目的研究染毒后鸡肉组织中二甲硝咪唑(DMZ)的残留情况,可能存在的原型药物及其自由代谢产物、结合残留物。方法利用生物酶解技术、化学技术对样品进行处理,得到游离态及结合态残留物组分,进一步采用高效液相色谱-电喷雾离子化-质谱(HPLC-ESI-MS)法检测其组成结构。结果在染毒动物鸡体12 h内肌肉组织样品中发现有原型药物DMZ(m/z 141),较多的游离羟基化代谢物2-羟基二甲硝咪唑(DMZO)(m/z 157)及少量的去甲基化游离代谢物2-甲基-5硝基咪唑(m/z127)的存在,未发现明显的葡萄糖苷酸化及磺化代谢物存在。结论DMZOH为DMZ的主要代谢物,未发现明显的结合态残留物存在。

Objective To study on the metabolites of Dimetridazole（DMZ） in the incurred chicken tissue, and to explore the possible existence of parent drug, free metabolites and bound residues. Methods The tissues were hydrolyzed by enzyme and chemicals to release residues, then extracts were explored by HPLC- ESI - MS. Results Residues including free parent drug DMZ（m/z 141）, major hydroxylated free metabolite DMZOH（m/z 157） and a minor demethylated free emtabolire 2 - methyl - 5 - nitroimidazole（m/z 127） were confirmed in the chicken muscle, which were collected within twelve hours after the chickens were stopped to be administered with DMZ. However, no evidence was obviously obtained for glucuronide and sulfated conjugations. Conclusion DMZOH was the major metabolite for DMZ, the bound residues were not be identified.