杭州市O139群霍乱弧菌耐药变迁及毒力基因携带

Variance of antibiotic resistance and virulence gene of Vibrio cholerae O139 in Hangzhou city

目的 研究杭州市1994～2003年分离的O139群霍乱弧菌耐药性变迁以及携带ctxA、tcpA毒力基因的情况。方法 运用K—B法和PCR，检测90株O139群霍乱弧菌对抗生索的敏感性以及是否携带ctxA、tcpA毒力基因。结果 90株O139群霍乱弧菌中无丁胺卡那耐药的菌株，13株对诺氟沙星和环丙沙星耐药；对氨苄青霉索、复方新诺明分别有5年和6年的耐药率为100％；2002、2003年耐药谱增加到7种。87．87％的O139群霍乱弧菌同时携带ctxA、tepA毒力基因。结论10年来分离自杭州的O139群霍乱弧菌的耐药情况日益严重；O139群霍乱弧菌是否携带ctxA、tcpA毒力基因在对抗生素敏感性方面差异有统计学意义。

Objective To investigate the variance of antibiotic resistance and the virulence gene ctxA and tcpA of Virbrio cholerae（VC） O139 strains in Hangzhou from 1994 to 2003. Methods 90 VCO139 strains were used to detect the antibiotic resistance and the virulence gene by disc diffusion test （Kirby-Bauer mehtod） and PCR. Results There was no amikacin-resistant strain and 13 strains were both norfloxacin-resistant and eiprofloxacin-resistnat. The 100 % antibiotic resis- tance happened to amplieillin in 5 years and SMZ-TMP in 6 years. 7 antibiotics were included in the resistance spectrum in 2002 and 2003. And the rate of the strains which carried both ctxA and tepA was 87.8 %. Conclusion The situation of increasing antibiotic resistance is serious. There is obvious difference of antibiotic resistance between VCO139 with ctxA, tcpA and VCO139 without etxA, tcpA.