超抗原SEB诱导外周血单核细胞杀伤肿瘤效果

Study on tumor-cytotoxicity effect of PBMC induced by super antigen SEB

目的通过观察葡萄球菌肠毒素B(SEB)对外周血单核细胞(PBMC)杀瘤活性的影响,寻找适用于肿瘤过继免疫治疗的新途径。方法密度梯度离心法分离富集PBMC,分别用10-12,10-11,10-10,10-9g/L浓度的SEB作用于PBMC,12,24,36,48 h后测定PBMC对K562细胞的杀伤活性。结果SEB处理PBMC 12 h后杀瘤活性增强,至24 h杀瘤活性最佳;在一定浓度范围内PBMC的杀瘤活性随SEB浓度的升高而增强。结论超抗原SEB在体外能够增强PBMC的杀瘤活性。

Objective Observing the tumor-cytotoxicity effect of periphera blood mononuclear cell（PBMC） induced by super antigen staphylococcus enterotoxin B（SEB）, so to find a new method in tumor adoptive immunotherapy. Methods Density grads centrifuge was used to separate and rich PBMC, then PBMCs were stimulated with different concentration of SEB, 10^-12g/L, 10^-11g/L, 10^-10g/L and 10^-9g/L. The tumor-cytotoxicity effect of k562 was detected after being induced 12, 24, 36 and 48 h. Results The tumor-cytotoxicity effect of PBMC began to inerease 12 h later, and the optimum time was 24 h. The tumor-cytotoxicity effect of PBMC is increasing with risement of SEB in a certain content. Conclusion Super antigen SEB does enhance the tumor-cytotoxicity of PBMC in vitro.