中药药对的化学成分研究——川芎赤芍挥发油的GC/MS分析

Chemical Component Study of Herb Pairs in Traditional Chinese Medicine—— Analysis of Volatile Oil in Rhizoma Ligustici Chuanxiong-Radix Paeoniae Rubra by GC-MS and Chemometric Resolution Method

中药药对是复方的最小组成单位,具有中药配伍的基本特点.药对化学是复方化学的核心内容.联用色谱和化学计量学方法为中药复杂体系的分离与分辨提供了强有力的工具.采用GCMS法分离测定中药药对川芎赤芍、单味药川芎和赤芍的挥发油成分,并对其中的重叠色谱峰采用化学计量学解析方法(CRM)进行分辨,得到药对和各单味药的纯色谱曲线和质谱图,药对川芎赤芍、单味药川芎和赤芍分别分辨出82,78和57个色谱峰,通过质谱库对分辨的纯组分进行定性,分别得到61,52和33个定性结果,占总含量的90.18%,95.14%和95.82%.

The volatile chemical components of herb pair Rhizoma Liguztici Chuanxiong-Radix Paeoniae Rubra （RLC-RPR） were analyzed by gas chromatography-mass spectrometry （ GC-MS）, chemometric resolution method （CRM）, and overall volume integration methods. By using these methods upon two-dimensional data, 52, 33, and 61 volatile chemical components in the essential oil of RLC, RPR, and herb pair RLC-RPR were, respectively, determined qualitatively and quantitatively, accounting for 95.14%, 95.82% and 90. 18% of the total contents of the essential oil of RLC, RPR and herb pair RLC-RPR, respectively. The experimental results show that the volatile chemical components of herb pair RLC-RPR in number are almost the addition of that of two single drugs RLC and RPR, but are changed in contents.