摘要
A series of oligomannuronates with series degree of polymerization（DP28） were prepared by acid hydrolysis from alginate-derived homopolymannuronate blocks, and the structures of the oligosaccharides were analyzed. The macromolecule of alginate was treated by partial acid hydrolysis and fractionated at pH2.85 resulting in homopolymannuronate blocks. The homopolymer was degraded by acid hydrolysis, and the oligosaccharides were separated and purified by gel chromatography. The oligosaccharides were characterized by fluorophore-assisted carbohydrate electrophoresis（FACE）, and the structures of derived oligomannuronates were determined by ESI-MS, NMR as well as FTIR analysis.
A series of oligomannuronates with series degree of polymerization （ DP = 2--8） were prepared by acid hydrolysis from alginate-derived homopolymannuronate blocks, and the structures of the oligosaccharides were analyzed. The macromolecule of alginate was treated by partial acid hydrolysis and fractionated at pH = 2.85 resulting in homopolymannuronate blocks. The homopolymer was degraded by acid hydrolysis, and the oligosaccharides were separated and purified by gel chromatography. The oligosaccharides were characterized by fluorophore-assisted carbohydrate electrophoresis（FACE） , and the structures of derived oligomannuronates were determined by ESI-MS, NMR as well as FTIR analysis.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第3期485-487,共3页
Chemical Journal of Chinese Universities
基金
国家'九七三'计划(批准号:2003CB716400)
国家'八六三'计划(批准号:2004AA621020)
山东省优秀中青年科学家科研奖励基金(批准号:03BS109)
中国科学院海洋研究所实验海洋生物学重点实验室开放基金(2004)资助.
关键词
褐藻胶
酸降解
甘露糖醛酸寡糖
制备
鉴定
Alginate
Acid hydrolysis
Oligomannuronate
Preparation
Identification
