摘要
采用反相高压液相色谱法,测定超滤过程中金银花中绿原酸含量与超滤体积的关系,结果表明1.25倍体积超滤基本上保留了有效成分。将超滤法与水提醇沉法对绿原酸的影响进行定性、定量及提取物得率的比较,结果表明两者斑点基本相同,但超滤法能更有效地保留有效成分。
The relationship of chlorogehic acid content in flos Lonicerae and ultrafiltration volume during ultrafiltration process was determined by reversed-phase high-pressure liquid chromatography.As a result,the active components were basically retained whan 1.25 times of original volume were ultrafiltrated.The comparative studies on effects of ultrafiltration,water-extraction and alcohol-precipitation on chlorogenic acid in qualitation,quantitation and extract yield indicated that both methods had same spots on TLC basically. But the former could retain the active components more effectivelly.
出处
《中成药》
CAS
CSCD
北大核心
1996年第2期4-6,共3页
Chinese Traditional Patent Medicine
关键词
高压液相色谱法
超滤
醇沉
金银花
绿原酸
reversed-phase high-pressure liquid chromatography,TLC,extract yield,ultrafiltration,alcohol-precipitation,Flos Lonicerae,chlorogenic acid