不同免疫抑制剂对血清转化生长因子β-1活性的影响

Elevation of Bioactive Transforming Growth Factor-β_1 in Serum from Renal Transplant Recipients Receiving Different Immunosuppressive Regimens

目的:测定肾移植术后血清转化生长因子β-1的活性,对比服用不同免疫抑制剂对转化生长因子β-1的活性的影响。方法:回顾分析肾移植患者的临床资料,术后采用环孢素A(CsA)预防排斥反应者25例(CsA组)、采用他克莫司(FK506)用药者20例(FK506组)。比较分析两组用药后血清转化生长因子β-1活性及总体转化生长因子β-1的浓度,并与正常人(12例)对比。结果:肾移植术后患者血清转化生长因子β-1的活性及总体转化生长因子β-1的浓度明显高于正常对照组(P<0·01),术后时间越长其增高越显著,以CsA组为甚,与FK506组比较存在显著性差异(P<0·05)。结论:肾移植术后患者血清转化生长因子β-1存在异常激活,可能参与慢性移植肾病的发病进程,术后采用免疫抑制剂FK506优于CsA。

Objective： To investigate the effects of different immunosuppressive regimens on circulating bioactive TGF-β1 serum levels in kidney transplant recipients. Methods： The bioactive TGF -β1 and the total TGF -β1 serum levels in kidney trans- plant recipients was detected by a well- established bioassay system using mink lung epithelial cell. Results： The bioactive TGF -β1 in kidney transplant recipients, as a group, had significantly higher levels compared with normal subjects （ P 〈0.01）. The serum total TGF - β1 levels in kidney transplant recipients was also higher （ P 〈 0.01 ）. Patients receiving CsA - based immunosuppression displayed significantly higher bioactive TGF- β1 levels and total TGF -β1 levels compared with those receiving FK506 - based immunosuppression （ P 〈 0.05）. Conclusion： TGF - β1 production is abnormal activated in kidney transplant recipients, maybe contributes to the development of chronic renal allograft nephropathy （CAN）.