高效液相色谱法测定人血浆中头孢曲松的含量

Determination of ceftriaxone in human plasma by HPLC

目的：建立人血浆中头孢曲松的高效液相色谱测定法（HPLC）。方法：血浆样品经10％三氯醋酸沉淀蛋白后，于ZORBAX Eclipse XDB-C18色谱柱（4．6mm×250mm，5μm）上，以含10mmol·L^-1戊烷磺酸钠的10mmol·L^-1。磷酸氢二钾缓冲溶液（以磷酸调节pH值为6．0）-乙腈（94：6）为流动相进行色谱分离，柱温为25℃，紫外检测波长为254nm。结果：线性范围0．2～10mg·L^-1，沉淀蛋白回收率81．6％～84．4%，加样回收率98．0％～100．5％，日内RSD2．27%～3．65％，日间RSD4．42％～5．13％。结论：本法准确可靠，可用于人血浆中头孢曲松的测定。

OBJECTIVE To establish an HPLC method for the determination of ceftriaxone in human plasma. METHODS 10% Trichloraeetie acid was used as deproteinizing agent for the plasma samples. The chromatographic separation was conducted on ZORBAX Eclipse XDB-C18 （4.6 mm×250 mm,5μm） column. The mobile phase consisted of acetonitrile-10 mmol·L^-1 phosphate buffer（6：94） containing 10 mmol·L^-1 sodium pentanesulfonat, adjusted to pH 6.0 with orthophosphoric acid. The temperature of column was at 25°C, the wavelength of detection was 254 nm. RESULTS The calibration curve was linear within 0.2-10 mg·L^-1. The deprotein recovery of ceftriaxone was 81.6%-84.4^. The recovery of methodology was 98.0%-100.5% with the with-day RSD of 2.27%-3.65%,and the between-day RSD of 4.42%-5.13%. CONCLUSION This method is accurate and reliable. It has been applied successfully to the determination of ceftriaxone in human plasma.