高速逆流色谱法分离制备丹酚酸B

Preparative Separation of Salvianolic Acid B from Salvia miltiorrhiza by High Speed Counter-Current Chromatorgraphy

采用高速逆流色谱法分离纯化丹参水溶性成分丹酚酸类物质,制备丹酚酸B化学对照品。分离采用的溶剂系统为正己烷-乙酸乙酯-水-甲醇(1.5:5:5:1.5),上相做固定相,下相做流动相,流速为1.7 mL/min,仪器转速850 rpm,进样量80 mg,纯度用HPLC方法测定。结果表明:一次分离可制备63.4 mg丹酚酸B,其纯度为98.6%。该方法操作简单,可作为高纯度丹酚酸B化学对照品的制备分离方法。

Salvianolic acid B, a major bioactive component in Danshen （ Salvia miltiorrhiza Bge. ）, was isolated and purified by high-speed counter-current chromatography （HSCCC） in semi-preparative scale from the crude extract of Danshen. Separation was performed with a two-phase solvent system composed of hexane-ethyl acetate-water-methanol （ 1.5 ： 5 ： 5：1.5, v/v） by eluting the lower aqueous phase at a flow-rate of 1.7 mL/min and a revolution speed of 850 rpm with injection volume of 80 nag. After the proceeding of separation,63.4 mg salvianolie acid B was obtained,and analyzed by normalization method according to HPLC areas giving a purity of 98.6% .HSCCC is a recommendable method to prepare and purify the active constituents from Salvia miltiorrhiza.