氯对紫外线灭活枯草芽孢杆菌的协同作用

Synergistic Disinfection of Bacillus subtilis Spores by UV Irradiation and Chlorine

以枯草芽孢杆菌为研究对象,研究了紫外线和氯单独及联合作用时的灭菌效果.结果表明,单独氯消毒时,对枯草芽孢杆菌的灭活效果很低,CT值为300(mg.min)/L时,灭活率仅为0.53个对数级;单独紫外线消毒对枯草芽孢杆菌有较好的灭活效果,紫外线剂量为40mJ/cm2时,对其有3.3个对数级的灭活效果.紫外线和氯联合消毒时,对枯草芽孢杆菌的灭活效果大大增强,当紫外线剂量为40mJ/cm2,氯的CT值为300(mg.min)/L时,对枯草芽孢杆菌的灭活率可达6.2个对数级,远高于二者单独作用效果之和.紫外线与氯的作用顺序对消毒效果有明显影响,以先紫外线照射后再加氯的效果最好.通过Berenbaum公式计算可知,紫外线与氯联合灭菌是一种协同作用,并且随着紫外线剂量及氯投量的增加,协同作用增强.

The inactivation effect of Bacillus subtilis spores was studied, both UV irradiation and chlorine disinfection individual and combined application process was examined with laboratory water samples. Results show that only 0.53 lg reduction was achieved by chlorine with CT value of 300（mg·min）/L, UV irradiation is more effective than that of chlorination, at a UV dose of 40mJ/cm^2 results in 3.3 lg reduction. Up to 6.2 lg reduction are achieved with a UV dose of 40mJ/cm^2 following by chlorine with CT value of 300 （mg·min）/L. The calculation from the Berenbaum formula verified that the effect of the combined applications of UV irradiation and chlorine in inactivatiing Bacillus subtilis is a kind of synergistic effect.