氯菊酯农药间接酶联免疫吸附测定法的建立

Indirect ELISA for the Pyrethroids-Permethrin Residue Detection

为测定环境样品中氯菊酯农药残留，用活泼酯法将氯菊酯半抗原（Py）与卵白蛋白（OVA）偶联，制备合成抗原Py-OVA作包被原，建立间接竞争酶联免疫吸附测定法．方阵滴定确定了抗血清最佳稀释度（1：2500），包被抗原的最适工作浓度0．45μg／mL，并建立了标准工作曲线．工作曲线表明在10-800μg／L浓度范围内呈良好的线性关系，回收率〉97％．

In order to detect the permethrin （Py） residue in environment samples, Ovalbumin（OVA）was used as protein carrier to couple with semi-antigen permethrin by active ester method. Indirect competitive ELISA （icELISA） was established. The most appropriate titration of coating antigen was 0.45μg/mL and optimal dilution was 1/2 500 correspondingly. The standard curve of icELISA was also established and the curve indicated that the lowest detection limit was 0. 1μg/mL, the curve had a favorable linear relation with the concentration range of 10-800μg/mL, recoveries of permethrin （ 〉 97 % ） were satisfactory.