一个寒证家系中发现15个差异表达基因的报告

A Report on 15 Differential Expression Genes Found in a Cold-Syndrome Genealogy

目的:精选家系切入证候研究,深层次地探讨寒证的分子生物学基础。方法:应用基因芯片技术对同一家系中的4例虚寒证患者与6名正常人进行基因表达谱检测。结果:发现此寒证家系中的虚寒证患者与能量等代谢相关的差异表达基因达15个,占此实验中已知功能基因的52%。结论:利用家系进行证候的基因组研究,可排除大量干扰因素,对于复杂的寒证可有效、集中地筛选目标基因,提示寒证家系患者机体能量异常、代谢减慢有相应的分子生物学基础。

To probe molecular biological basis of cold syndrome. Methods ： Microarray technique was used for examination of the gene expression profiles in 4 cases of cold syndrome patient and 6 normal persons in a same family. Results： 15 differential expression genes asociated with energy metabolism were found in the patients of deficiency cold syndrome in the cold syndrome family,accounting for 52% of the known functional groups in this experiment. Conchsion.Using genealogy to study genoms of syndromes can eliminate a lot of interfering factors, and is benefit to effectively screen target genes for complicated cold- syndrome ,suggesting that cold-syndrome family has corresponding molecular biological basis for lower energy metabolism.