中药抗疲Ⅰ号对训练Wistar大鼠急进高原并运动力竭后肝脏组织及其酶谱的影响

The Effect of Anti- Fatigue Ⅰ (抗 疲1号), the Chinese Drugs Preparation,on Hepatic Tissue and the Zymogram of Wistar Mice Trained to Make Rush Entry into Plateau and into Post-exercise Exhaustion

目的通过训练大鼠急进高原模型从肝脏组织形态改变和血中酶谱水平研究训练对大鼠肝脏组织的损伤及防护。方法在海拔1520m环境设实验组(EG)使用中药抗疲Ⅰ号加入常规饲料中喂养,对照组(CG)常规饲料喂养。EG、CG各40只游泳训练4周后,各选30只3h急进至海拔3850m环境静息1.5h,从中各选10只作为静息对照采集标本,EG、CG各20只游泳至力竭后,1、24h采集标本。结果EG与CG相比,血中酶谱含量差异均有显著性。EG肝组织结构正常,肝细胞索结构清晰,核清晰,无变性;CG肝组织结构紊乱,肝细胞索模糊。结论过度训练、改变运动集训环境或进入高海拔地区作业训练更要特别注重加强劳动和训练防护。本防护模型研究通过食品干预训练大鼠,是一种简便易行的方法。

Objective:To study training- caused hepatic tissue damage and its protection by training Wistar mice to make rush entry into plateau in order to observe the form change of hepatic tissue and blood zymogram level.Method:At the altitude of 1520ms,one experimental group(EG)of 40 mice were fed with a mixed feed with Anti- fatigue Ⅰ ,(抗 疲 1号 ),the Chinese Drugs Preparation,while one control group(CG)of the same number were fed with the routine feed.After both groups were trained to swin for 4 weeks,30 mice in each group were chosen to make rush entry into 3850- meter altitude within 3 hours,then they were allowed to rest for 1.5 hours,and then 10 from 30 mice in each group were chosen as rest group for comparison,and when 20 mice in each group were forced to swim until exhaution,they were sampled 1 hour later and 24 hours later.Result Comparison between EG and CG shows a significant difference in blood zymogram content.EG hepatic tissue loods normal;hepatic trabecula structure looks clear and hepatic cell nucleus also look clear with no change in nature.On the contrary,CG hepatic tissue is in disorder with blurred hepatic trabecula etc.Conclusion:The resulet suggests a slower restoration of zymogram in 24 hours for unprotected mice traning model CG,which is related to some unbalanced multi- links of individual inner environment and needs further systematical analysis and study. The change of CG hepatic tissue hints that its mechanism is inferior to EG's,accompanied by pathological damage,and also reveals the mechanism which can raise zymogram.It is concluded that much more attention should be paid to the protective laboring and training when intensive training or intensive laboring are preformed or undertaken,especially in the sudden changed environment or at altitudes.This protection model,by food intervention in training mice,is a simple and easy way of such protection.