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绵羊肺腺瘤病毒JSRV-2基因探针的制备与原位杂交检测 被引量：3

Preparation of JSRV-2 DNA probe and detection by in situ hybridization of Jaagsiekte sheep retrovirus
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摘要 用地高辛随机引物法标记外源性exJSRV特异的JSRV-2片段,制备探针,用原位杂交法检测自然感染绵羊肺腺瘤病(OPA)的病肺组织中JSRV-NM的RNA及前病毒DNA,结果表明OPA患羊肺肿瘤细胞的胞浆和核内都有JSRV-2基因mRNA的表达,同时也检测到了前病毒DNA,而相应的阴性对照无阳性信号,证实外源性JSRV-NM病毒具有特异性的DNA探针在检测致瘤性前病毒在宿主细胞中的整合具有可信度。 The exJSRV-2 specific fragment DNA probe was labeled with digoxigenin （Dig）, Jaagsiekte retrovirus NM strain RNA and proviral DNA in the lung tissue of OPA were detected by in situ hybridization （ISH）. The results showed JSRV-NM mRNA and provirus DNA in sheep lung tumor cells had very strong hybridization signals and the control group has no positive signals.
作者 刘淑英 齐景伟 马学恩
机构地区 内蒙古农业大学动物科学与医学学院
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2006年第2期208-211,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金资助项目(批准号30260083和30560108)
关键词 绵羊肺腺瘤病毒 JSRV-2基因 探针 原位杂交 Jaagsiekte sheep retrovirus JSRV-2 gene probe in situ hybridization
分类号 S852.65 [农业科学—基础兽医学]
  • 相关文献

参考文献11

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二级参考文献40

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共引文献33

同被引文献38

  • 1杨惠,刘淑英.利用JSRV构建啮齿动物LPA模型研究进展[J].中国农业大学学报,2019,24(11):98-105. 被引量：2
  • 2梁化春,邢晋凌,刘淑英,徐萌杰.绵羊肺腺瘤病实验室诊断报告[J].内蒙古农业大学学报（自然科学版）,2008,29(4):119-124. 被引量：5
  • 3赵泽赞.绵羊肺腺瘤病毒的分子生物学技术检测[D].呼和浩特:内蒙古农业大学基础兽医学,2011.
  • 4刘淑英,齐景伟,徐萌杰,等.绵羊肺腺瘤病毒的斑点杂交技术检测[A].中国畜牧兽医学会兽医病理学分会、中国病理生理学会动物病理生理专业委员会.中国畜牧兽医学会兽医病理学分会第十六次学术研讨会、中国病理生理学会动物病理生理专业委员会第十五次学术研讨会论文集[C].泰安:中国畜牧兽医学会兽医病理学分会、中国病理生理学会动物病理生理专业委员会,2009.
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引证文献3

二级引证文献8

中国预防兽医学报
2006年 第2期

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