多聚酶链反应区分血清Ⅰ型MDV强弱毒株

Differentiation of Pathogenic and Non-pathogenic Serotype ⅠMarek's Disease Virus by the PCR DNA Amplification

选用血清Ⅰ型马立克氏病病毒（ＭＤＶ＿１）基因组中位于１３２ｂｐ正向重复序列两侧的２段寡聚核苷酸为多聚酶链反应（ＰＣＲ）的引物，分别对Ⅰ型ＭＤＶ强毒株（京－１株）和弱毒株（Ｍｄ１１／７５Ｃ株）的核酸进行基因扩增。结果显示：该弱毒株核酸基因中含６个或更多个拷贝数的１３２ｂｐ正向重复序列，而强毒株核酸基因中仅含２个，相同引物对Ⅱ型、Ⅲ型ＭＤＶ（ＳＢ－１株，Ｆｃ１２６株）和禽网状内皮组织增生病病毒（ＲＥＶ）核酸作ＰＣＲ扩增，未检出任何核酸片段。

Differentiation of pathogenic and non-pathogenic strains (Beijing-1,Md11/75℃) of Marek's disease virus typeⅠ(MDVⅠ) was attempted by polymerasechain reaction (PCR) using the primers chosen to flank the 132-base-pair tandem directrepeats in the serotypeⅠMDV genome.The PCR reaction was specific for serotypeⅠMDVs, amplifying fragments corresponding to two copies of the tandem repeats presentin Bejing-1 and six or more copies on Md11/75℃ virus.No DNA was amplified fromDNA extracts in cells infected by SB-1 and Fc126 strains and reticuloendotheliosis virus.