mPEG修饰红细胞解决AIHA患者配血困难的体外研究

In vitro modification of RBC with mPEG-SPA for resolving the problem of difficult blood cross match of AIHA patients

目的探讨mPEG-SPA修饰对红细胞结构功能的影响,以及修饰O型红细胞解决AI HA患者疑难配血的可能性。方法对比2.0mmol/L mPEG-SPA修饰前后RBC保存期内的结构和功能指标,采用抗球蛋白法对比修饰前后的配血结果,采用流式细胞术观察mPEG-SPA修饰对自身抗体的遮蔽效果。结果保存期内2.0mmol/LmPEG-SPA修饰RBC对其结构功能无影响,配血结果表明2.0mmol/L mPEG-SPA修饰的RBC符合临床用血要求,流式检测结果显示mPEG-SPA修饰对自身抗体有很好的遮蔽效果。结论mPEG修饰RBC有可能为解决AI HA患者配血困难开辟一条实用有效途径。

Objective To observe the effect of mPEG-SPA on RBCs and to study the possibility of resolving the problems of difficult blood cross match among AIHA patients. Methods Both the structure and functions of donor RBC before and after mPEG-SPA camouflage were tested and were compared during the period of RBC storage. Antiglobulin cross mateh of red cells were performed before and after the mPEG-SPA camouflage. The camouflage effect on autoantibodies were studied by flow cytometry. Results Modification of donor RBCs by 2.0mmol/L mPEG-SPA did not influence its structure and functions during the storage period. Flow cytometry demonstrated that the modification had good camouflage effect on autoantibodies. Conclusion The modification of RBCs with 2.0mmol/L mPEG- SPA provides a useful strategy for resolving the problems of blood eross match for AIHA patients.