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进境小麦中沙地牧草腥黑粉菌的鉴定 被引量：1

Identification of Tilletia ehrhartae from imported Australian wheat
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摘要 从上海口岸进境的澳大利亚小麦中发现一种类似小麦印度腥黑粉病菌的腥黑粉菌冬孢子,对该菌冬孢子进行了形态学特征和PCR检测,根据结果,将这种冬孢子鉴定为沙地牧草腥黑粉菌Tilletiaehrhartae;本研究设计了T.ehrhartaea的特异引物Eh2/Eh4,结合引物Till/Til4建立了T.ehrhartae的套式PCR检测方法。 Teliospores similar to Tilletia indica were found in imported wheat from Australia and identified as T. ehrhartae based on the morphological characteristics and PCR analysis. This tuberculate teliospore was described. A nested PCR method was developed to detect teliospore of T. ehrhartae with primers Eh2/Eh4 and Till/Til4 designed form ITS sequence of T. ehrhartae .
作者 刘素萍 易建平 周国梁 叶军 印丽萍
机构地区 上海出入境检验检疫局
出处 《植物检疫》 2006年第1期7-9,共3页 Plant Quarantine
基金 上海市重大科技攻关项目(03DZ19315和04DZ05002) 国家质检总局科技专项(2004IK046)的资助。
关键词 沙地牧草腥黑粉菌 套式PCR 鉴定 Tilletia ehrhartae, nested PCR, identification
分类号 S435.121.4 [农业科学—农业昆虫与害虫防治]
  • 相关文献

参考文献10

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二级参考文献24

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共引文献42

同被引文献22

  • 1易建平,刘素萍,印丽萍,周国梁.TaqMan-MGB探针在小麦印度腥黑穗病菌和黑麦草腥黑穗病菌鉴定上的应用[J].植物检疫,2005,19(1):15-19. 被引量：14
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  • 3Castlebury LA, Carris LM, Tilletia walkeri, A new species on Lolium multiflorum and L . Perenne . Mycologia , 1999,91 : 121 - 131.
  • 4Bonde MR,Peterson GL, Schaad NW, et al. Karnal bunt of wheat. Plant Dis, 1997 ,81:1370 - 1377.
  • 5Smith OP, Peterson GL, Beck R J, et al. Development of a PCRbased method for identification of Tilletia indica, causal agent of karnal bunt of wheat. Phytopathology, 1996,86( 1 ) : 115 - 122.
  • 6Frederick RD, Karen ES, Tooley PW, et al. Identification and differentiation of TiUetia indica and T. walkeri using the polymerase chain reaction. Phytopathology ,2000,90(9) :951 - 960.
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  • 10Mishra A,Singh US,Goel R, et al. PCR based molecular technique for identification and discrimination of quarantined and nonquarantined TiUetia sps. Indian J Exp Bial, 2002,40 ( 10 ) : 1137 - 1142.

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植物检疫
2006年 第1期

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