丹参提取制剂对耳蜗毛细胞琥珀酸脱氢酶的影响

Effects of salvia miltiorrhizae injection on succinate dehydrogenase in the cochlear hair cells of the guinea pigs

目的:观察具有活血化淤,扩张耳蜗血管,改善内耳微循环之功效的丹参提取制剂对豚鼠庆大霉素耳中毒引起的耳蜗毛细胞琥珀酸脱氢酶变化的影响。方法:实验于2004-09/12在泰山医学院生理听觉研究室完成。选用耳郭反射正常、体质量为250～300g的健康杂色豚鼠,雌雄不拘,排除耳郭反射的声刺激强度超过正常±2.5dB的实验豚鼠。将动物随机数分成3组:正常对照组动物15只,肌肉注射生理盐水2mL/(kg·d);庆大霉素组动物15只,肌肉注射庆大霉素80mg/(kg·d);丹参注射液组动物15只,腹腔注射丹参注射液(含生药量1.5kg/L)6mg/(kg·d),然后肌肉注射庆大霉素同庆大霉素组。各组动物连续注射药物均20d。用脑干听觉诱发电位的方法检测庆大霉素耳中毒豚鼠的听阈变化;用组织化学方法检测耳蜗毛细胞琥珀酸脱氢酶的变化。结果:在实验过程中无动物死亡,进入结果分析3组动物共45只。①用药22d后,庆大霉素组、丹参注射液组豚鼠的脑干听觉诱发电位反应阈值与正常对照组比较,差异有显著性犤(71.25±24.730),(41.05±10.930),(34.65±1.321)dB;t=8.097,3.184,P<0.01犦;丹参注射液组与庆大霉素组比较,差异有显著性(t=6.118,P>0.01)。②耳蜗铺片毛细胞内琥珀酸脱氢酶染色在光镜下观察,正常豚鼠耳蜗基底膜铺片毛细胞琥珀酸脱氢酶染色呈紫蓝色;显色分布均匀,毛细胞排列整齐;在庆大霉素组耳蜗毛细胞琥珀酸脱氢酶显色出现明显消失,耳蜗毛细胞破坏明显;丹参注射液组琥珀酸脱氢酶染色变化较轻,庆大霉素组与丹参注射液组耳蜗铺片显示有明显差异。结论:丹参注射液能降低庆大霉素对耳蜗毛细胞的损害作用,保护耳蜗线粒体呼吸酶的活性,维持毛细胞的能量代谢以供给细胞需要能量的功能活动,可能是丹参注射液降低庆大霉素耳毒性的机制之一。

AIM： To investigate the effects of salvia miltiorrhizae injection （SM） on suecinate dehydrogenase （SDH）＇in the cochlear hair cell of the guinea pigs with ototoxieity induced by gentamicin （GM）. METHODS： The experiment was completed at the Heating Research Room of Taishan Medical College from September to December 2004. Healthy mottle guinea pigs with nomal auricle reflex （250-300 g in body mass, of either gender） were randomly divided to 3 groups with .15 in each group： control group with intramuscular saline injection [2 mL/（kg·d）]; GM group with intramuscular gentamicin injection [80 mg/（kg·d）]; SM group with gentamicin as the same as the GM group after SM [6 mg/（kg·d） containing 1.5 kg/L raw drug] being injected intraabdominally. All three group were administrated for 20 days. The changes of auditory reaction threshold and SDH in the cochlear hair cells were detected by brainstem auditory evoked potential （BAEP） and histochemistry respectively. RESULTS： Totally 45 animals in 3 groups entered the final analysis. ① The thrcsholds of BAEP reaction in the control group, the GM group and the SM group before administration were significantly different from those 22 days after administration [（71.25±24.730）, （41.05±10.930）, （34.65±1.321） dB; t=8.097,B.184,P 〈 0.01]; there was a significant difference hetween SM group and GM group （t=6.118, P〉 0.01）. ② The change of SDH in the cochlear hair cells： The SDH histochemical staining a purple-blue color could be observed with the light microscope in the surface preparation of normal guinea pigs cochlear basal membrane and the hair cells were arranged in good order and were even in color. The SDH chemical reaction color obviously disappeared in cochlear hair cells of guinea pigs in GM group, there was a light change of the SDH level in SM group. There was a significant difference between the two groups. CONCLUSION： SM can reduce the injury of the cochlear hair cells induced by GM and protect the vitality of mitoehondrial respiratory enzyme in the cochlear hair ceils, which may be a possible mechanism of action of SM therapy.