疏解少阳和祛风止痛法对大鼠体外血栓形成及血小板聚集功能的影响(英文)

Effects of the methods of treating Shaoyang by dispersing and dispelling wind and relieving pain on thrombosis in vitro and function of platelet aggregation in rats

背景:偏头痛病位多在少阳经循经之处,以疏解少阳、祛风止痛法从少阳胆经治疗偏头痛疗效显著,但该两法对体外血栓形成及血小板聚集功能的作用又如何呢?目的:通过大鼠体外血栓形成实验,大鼠血小板聚集功能实验,探讨疏解少阳法与祛风止痛法对两者的影响,并通过拆方配伍实验,探讨疏解少阳法与祛风止痛法的配伍意义。设计:完全随机分组设计,对照实验。单位:黑龙江中医药大学基础医学院方剂学教研室。材料:实验于2000-03/08于黑龙江中医药大学方剂学方药实验室完成。选用健康成年Wistar大鼠60只。随机将大鼠分为6组:正常对照组、阳性对照组,全方高剂量组,全方低剂量组,疏解少阳组,祛风止痛组,每组10只。方法:①阳性对照组:按0.39g/kg剂量灌胃0.034g/mL复方羊角片(哈尔滨生物化学制药二厂生产)混悬液;全方(全方剂成分:柴胡20g,黄芩10g,半夏15g,甘草10g,川芎20g,天麻15g,细辛5g,全蝎5g,蜈蚣5g)高、低剂量组:按17.40,8.70g/kg剂量灌胃1.5,0.75g/mL全方剂;疏解少阳组(疏解少阳剂成分:柴胡20g,黄芩10g,半夏15g,甘草10g)和祛风止痛组(祛风止痛剂成分:川芎20g,天麻15g,细辛5g,全蝎5g,蜈蚣5g):按8.70g/kg剂量灌胃0.75g/mL疏解少阳剂和祛风止痛剂;正常对照组:灌胃等体积生理盐水。各组连续干预12d。②体外血栓形成实验:末次给药后2h,分离一侧颈总动脉。结扎远心端,并在近心端以动脉夹阻断血流,将动脉插管插入颈总动脉内。松开动脉夹,取1.8mL血液入一血栓形成仪的旋转环内,以17r/min旋转15min。停止转动后,倒出形成的血栓,测量血栓长度、血栓湿重。将湿血栓烘干,测量血栓干重。③血小板聚集功能测定:采用比浊测定法记录富血小板血浆在1,5min的聚集率,5min内最大聚集率及聚集抑制率。④计量资料比较采用t检验。主要观察指标:疏解少阳法和祛风止痛法对体外血栓形成及血小板聚集功能的影响。结果:进入结果分析大鼠60只。①全方高、低剂量组和阳性对照组及祛风止痛组的体外血栓长度、湿重、干重明显低于正常对照组(P<0.05～0.01)。全方低剂量组的血栓湿重及干重明显低于疏解少阳组(P<0.05)。②各用药组的大鼠血小板1min聚集率、5min聚集率、最大聚集率均明显低于正常对照组(P<0.05～0.01)。且阳性对照组和全方高、低剂量组及祛风止痛组作用效果强于疏解少阳组。结论:单用疏解少阳方无明显抗体外血栓形成和抑制血小板聚集的作用,祛风止痛方和疏解少阳合用之后作用明显增强。

BACKGROUND： Migraine is usually located in the course of Shaoyang channel, and the curative effect of treatment with methods of treating Shaoyang disease by dispersing and dispelling wind （DW） as well as relieving pain （RP） on migraine is prominent, but how about the effects of these two ways on thrombogenesis in vitro and the function of platelet aggregation （PA）？ OBJECTIVE： To probe into the effect of treatment of Shaoyang disease and methods of DW and RP on thrombogenesis in vitro and function of PA through＇the experiments of thrombogencsis in vitro and function of PA in rats, and investigate the compatible significance of DS and DW as well as RP through formula compatible experiment. DESIGN： Completely randomized grouping design and controlled trial. SETTING： Department of Prescription, College of Basic Medical Sciences, Heilongjiang University of Chinese Medicine. MATERIALS： The experiment was conducted in the Laboratory of Department of Prescription, Heilongjiang University of Traditional Chinese Medicine from March to August 2000. Sixty heahhy aduh Wistar rats were randomly divided into 6 groups： Normal control group, Shaoyang treatment group （groupⅠ ）, DW and RP group （group Ⅱ ）, high-dose and whole-prescription group （group Ⅲ）, low-dose and whole-prescription group （group Ⅳ）, and positive control group with 10 rats in each group. METHODS： ① Positive control group： Gastric perfusion of 0.034 g/mL compound divaside slice suspension （made by Harbin Second Biochemical Pharmacy co., Ltd） was given to rats （0.39/kg）; Group Ⅲ and IV （ingredients： Chaihu 20 g, huangqin 10 g, banxia 15 g, gancao 10 g, chuanqiong 20 g, tianma 15 g, xixin 5 g, quanxie 5 g, wugong 5 g）： Gastric perfusion of 1.5, 0.75 g/mL of complete prescription was given to rats （17.40, 8.70 g/kg）; Group Ⅰ （ingredients： Chaihu 20 g, huangqin 10 g, banxia 15 g, gancao 10 g） and group Ⅱ （ingredients： Chuanqiong 20 g, tianma 15 g, xixin 5 g, quanxie 5 g, wugong 5 g）： Gastric perfusion of 0.75 g/mL agents of treatment with DS and DW wind antidyne was given to rats as 8.70 g/kg; Normal control group： Gastric perfusion of normal saline of the same volume was given to rats. Intervention on rats of all groups lasted 12 days. ②Experiment of thrombogenesis in vitro： The cephalic artery of one side was seperated 2 hours after the last time of administration. The distal part was deligated, the blood current in proximal part was blocked with bulldog clamp and the arterial cannula was inserted into the cephalic artery. Loosened the bulldog clamp, blood of 1.8 mL was collected and put into the rotary ring of thrombogensis meter to rotate for 15 minutes at 17 r/min, and then poured the thrombus, measured the length and humid weight of thromb. After that, dried the humid thromb and measured the dry weight. ③ Determination of PA： The packing fraction （PF） in the 1^st and 5^th minutes as well as the maximal packing fraction （MPF） and assembling inhibition ratio of platelct-rich plasma within 5 minutes were recorded with nephelometry. ④ Differences of measurement data were compared with t test MAIN OUTCOME MEASURES： Effect of treating Shaoyang disease and methods of DW as well as RP on thrombogenesis in vitro and thefunction of PA.RESULTS： A total of 60 rats were involved in the analysis of results. ① The length, humid weight and dry weight of thrombogenesis in vitro in group Ⅲ and Ⅳ, positive control group and group Ⅱ were obviously lower than those in the normal control group （P 〈 0.05-0.01）; The humid weight and dry weight of thromb in group Ⅳ were significantly lower than those in group Ⅰ （P 〈 0.05）. ② The PF in 1^st, 5^th minute and the maximal aggregation （MA） of blood platelet in rats of each group were significantly lower than those in the normal control group （P 〈 0.05-0.01）, and the curative effect on rats in the positive control group, group Ⅲ and Ⅳ as well as group Ⅱ were better than that in group Ⅰ, CONCLUSION： There are no significant effects of dispersing thrombogenesis in vitro and inhibiting PA by only treating Shctoyang disease. The effect of combined prescription of DW and RP as well as treatment of Shaoyang disease are obviously enhanced.