三氧化二砷对鼻咽癌细胞周期和细胞骨架微丝的影响

The effects of arsenic trioxide on the cell cycle and microfilament cytoskeleton in human nasopharyngeal carcinoma cell line

目的研究三氧化二砷(As2O3)对鼻咽癌细胞周期和细胞骨架微丝的影响并探讨其作用机制。方法采用流式细胞仪(flowcytometry,FCM)、激光扫描共聚焦显微镜(laserscanningconfocalmicroscopy,LSCM)和荧光标记技术,检测人鼻咽癌细胞株(nasopharyngealcarcinomacellline,CNE1)经As2O3诱导后细胞周期和细胞骨架微丝的变化。结果FCM显示,经浓度为2μmol/L、4μmol/LAs2O3处理后,G1期的细胞显著增加(P<0.001);经8μmol/LAs2O3处理后,出现明显的细胞凋亡峰(P<0.001),S和G2/M期细胞显著增加(P<0.001)。经4μmol/L、8μmol/LAs2O3处理后,细胞内纤维型-肌动蛋白(F-actin)含量明显减低(P<0.001)。LSCM图像观察到,经4μmol/LAs2O3处理后,标记F-actin的绿色荧光显著减弱;经8μmol/LAs2O3处理后,凋亡细胞具有典型的凋亡形态特征。结论As2O3引起的细胞周期的改变对CNE1细胞的分化和凋亡起重要作用;As2O3诱导的细胞骨架微丝的改变与细胞周期的改变密切相关。

OBJECTIVE To evaluate the effects of arsenic trioxide （As2O3） on the cell cycle and microfilament cytoskeleton in human nasopharyngeal carcinoma cell line （CNE1）, as well as possible mechanisms. METHODS The variation of cell cycle and microfilament cytoskeleton in CNE1 were observed using the flow cytometry （FCM）, the laser scanning confocal microscopy （LSCM） and technology of fluorescence.RESULTS FCM showed that the proportion of G1 phase cells significantly increased in cells exposed to 2 and 4 μ mol/L As2O3（P〈0.001）. Apoptotic peak which was detected after treated with 8μmol/L As2O3 was significantly higher than that either treated with 2 and 4 μmol/L As2O3 or untreated with As2O3 （P〈0.001）.Proportion of S phase and G2/M phase cells significantly increased in cells exposed to 8 μ mol/L As2O3（P〈0.001）. The value F-actin in CNE1 treated with 4 and 8 μ mol / L As2O3 was significantly reduced than that either treated with 2 lu mol/L As2O3 or untreated with As2O3 （P〈0.001）. The scanning images （LSCM） displayed that the fluorescence signal of F-actin in CNE1 that treated with 4 and 8μmol/L As2O3 was significantly decreased than that either treated with 2 μmol / L As2O3 or untreated with As2O3. CNE1 cells that treated with 8μmol/L As2O3 appeared typical morphological features of apoptosis. CONCLUSION As2O3 can influence the cell cycle of CNE1 and play an important role in the cell differentiation and apoptosis. The change of the cell microfilament cytoskeleton induced by As2O3 is closely related to the change of the cell cycle.