腹腔镜胃癌根治术后腹腔冲洗液CEA mRNA的变化和意义

Changes of CEA mRNA in peritoneal lavage after laparoscopy-assisted radical gastrectomy and its clinical significance

目的探讨腹腔镜胃癌根治术对腹腔脱落癌细胞的影响。方法收集44例行腹腔镜胃癌根治术患者胃癌切除前后腹腔冲洗液,并以RT-PCR方法检测腹腔冲洗液CEA mRNA表达情况。结果腹腔镜胃癌根治术患者CEA mRNA表达胃癌未切除时阳性率为27.3%(12/44),切除后阳性率为36.4%(16/44),两组之间差异无统计学意义(P>0.05)。结论腹腔镜胃癌根治术不增加腹腔胃癌细胞的脱落。

Objective To investigate the effect of laparoscopic-assisted radical gastrectomy on gastric cancer cells in peritoneal lavage fluid from patients＇ with gastric cancer. Methods Peritoneal lavage fluid from 44 patients with gastric cancer was collected before and after laparoscopic-assisted radical gastrectomy to detect carcinoembryonic antigen （CEA） mRNA expression using RT-PCR. Results The pre-, and postoperative CEA mRNA positive rate was 27.3% （12/44） and 36.4% （16/44）, respectively, and there was no significant difference （ P 〈0.05）. Conclusions Laparoscopic-assisted radical gastrectomy doesn＇t increase the intraperitoneal free cancer cells.