弓形虫SAGⅠ基因的克隆与原核表达

Cloning and Prokaryotic Expression of Toxoplasma gondii SAGⅠ Gene

利用PCR技术从弓形虫RH株基因组中扩增出SAGⅠ部分基因片段,亚克隆入表达载体pGEX-KG,将重组质粒转化入大肠杆菌BL21,IPTG诱导表达,SDS-PAGE电泳显示表达的重组蛋白的分子量为56ku。免疫印记分析显示此表达产物能被猪抗弓形虫阳性血清所识别,表明该重组蛋白具有免疫反应活性。为进一步进行弓形虫病的免疫预防和诊断研究奠定了基础。

The truncated SAG Ⅰ gene was amplified by PCR and subcloned into a prokaryotic expression vector PGEX-KG and transformed into E. coli BL21. After introduction by IPTG, high expression was found by SDS-PAGE and the molecular weight of recombinant fusion protein was approximately 56 ku. The results of western-blot indicated that recombinant protein was recognized by specific antibody. This result will be useful for further research on the prevention and diagnose of toxoplasmasis.