常青胶囊抗弓形虫速殖子实验研究

In vitro Effect of Combined Traditional Chinese Medicine (Changqing Capsule) on The Tachyzoites of Toxoplasma gondii

目的观察常青胶囊体外抗弓形虫速殖子的效果。方法常青胶囊设高、中、低3个剂量组。用生理盐水浸泡不同剂量的常青胶囊,各取上清液1·5ml,加入2·5×104弓形虫速殖子,作用8h后,将速殖子腹腔接种及灌胃接种正常小鼠,观察发病情况。如不发病,同法接种传代观察发病情况,至第3代。抗弓形虫药物螺旋霉素、乙胺嘧啶及阿奇霉素3药同样各设高、中、低3个剂量组。另设生理盐水对照组。腹腔接种各药物作用的速殖子后观察小鼠发病情况。结果常青胶囊腹腔接种组发病数(60只小鼠中2只发病),显著低于上述3药对照组及生理盐水对照组(60只小鼠发病数依次为16、10、10及60只)(P值均<0·05)。常青胶囊高、中剂量组(每组20只小鼠,发病数均为0)与低剂量组(20只小鼠,发病2只)之间差异显著(P<0·05)。传代观察,常青胶囊低剂量组腹腔及灌胃接种传第1代,20只小鼠分别有2只及1只发病。螺旋霉素低剂量组传第2代,20只小鼠中2只发病,乙胺嘧啶低剂量组传第3代,20只小鼠有1只发病。结论常青胶囊体外抗弓形虫作用优于传统临床抗弓形虫药物,杀虫效果与剂量呈正相关。

Objective To detect the in vitro effect of the traditional Chinese medicine on the tachyzoites of Toxoplasma gondii. Methods Supernatant （ 1.5 ml） of different doses of the traditional Chinese medicine （ Changqing capsule） was collected by normal saline immersion and 2. 5 × 104 Toxoplasma gondii tachyzoites were added in each paste well for 8 hours. Spiramycin, pyrimethamine and azithromycin in different doses were used as controls. Normal saline was used as negative control. Mice were inoculated with drug-treated tachyzoites intraperitoneally or intragastrically. The normal mice were subcultured after 8 days for 3 generations. Results The incident number of the infected mice was significantly different among groups with different drugs and doses： 2/60, 16/60, 10/60 and 10/60 in the groups of Changqing capsule, spiramycin, pyrimethamine and azithromycin respectively （ P 〈 0. 05 ）. No mice were found incident in groups of high and medium dose Changqing capsule while 2 out of 20 found sick in the low dose group （ P 〈 0. 05 ）. The subculture observation showed that 2 and 1 mice in the first generation of the low dose Changqing capsule group inoculated intraperitoneally and intragastrically were found infected respectively. 2 mice of the second generation in low dose spiramycin group and 1 mouse of the third generation in low dose pyrimethamine group were also found infected. Conclusion The in vitro killing effect of the Changqing capsule on the tachyzoites of Toxoplasma gondii is better than the current clinical drugs and shows a positive correlation with the dosages.