中药鸦胆子与补骨脂素对卡氏肺孢子虫病大鼠免疫调节的影响

Immunological Regulation and Treatment of Brucea javanica and Fructus Psoraleae on Rats with Pneumocystis carinii Pneumonia

目的探讨中药鸦胆子和补骨脂素合剂对卡氏肺孢子虫肺炎(PCP)大鼠的免疫调节及杀虫作用。方法地塞米松皮下注射SD大鼠(2·5mg/只,每周2次,连续6周),建立PCP大鼠模型。药物治疗剂量,每鼠每天口饲中药鸦胆子0·12mg与补骨脂素1mg,连续7d或14d。同时设PCP阳性不治疗对照组及正常大鼠对照组。制作肺组织病理切片观察肺组织病变,制作肺组织印片计数肺孢子虫包囊数观察中药合剂杀虫效果。检测血液中CD4+T细胞、CD8+T细胞和α肿瘤坏死因子(TNF-α)的变化,观察中药合剂对PCP大鼠的免疫调节作用。结果治疗组大鼠,受损的肺组织病变减轻或修复,体重明显回升,肺组织包囊数明显低于阳性对照组(P<0·05)。血液中CD4+T细胞、CD8+T细胞和TNF-α均较阳性对照组升高(P<0·05)。结论中药鸦胆子和补骨脂素合剂治疗PCP大鼠,可增强免疫调节作用,并可抑制及杀灭卡氏肺孢子虫。

Objective To study the imumunological regulation and treatment of Brucea javanica and fructus psoraleae, traditional Chinese medicine, on rats with Pneumocystis carinii pneumonia （PCP）. Methods Rats were injected subcutaneously by dexamethasone. When the rats got Pc infected, they were divided into two groups： rats in one group were treated with the mixture of Brucea javanica and fructus psoraleae and another group was used as infected control. Control with normal rats was also established. Observations were made on the number of cysts in lungs and the changes of CD4^＋ T cells, CD8^＋ T cells and TNF-α in the serum to demonstrate the imumunological regulation and killing effect of the medicine on cysts in the infected rats. Results The body weight of the rats treated with Brucea javanica and fructus psoraleae increased considerably than that of immunosuppressed rats and the normal control. The damaged lung got improved and repaired, and a significant cyst reduction was shown in the treated group. The CD4^＋ T cells, CD8^＋ T cells and level of TNF-α in serum also increased in the treated group significantly. Conclusion The mixture of Brucea javanica and fructus psoraleae plays an imumunological regulation on rats with Pneumocystis carinii pneumonia and shows certain killing effect on the cysts.