表皮生长因子受体抑制剂AG1487对舌鳞状细胞癌细胞Tca8113细胞外信号调节激酶抑制作用的研究

The inhibitory effect of epidermal growth factor receptor inhibitor AG1487 on extracellular signal-regulated kinase of Tca8113 cells

目的了解表皮生长因子受体抑制剂AG1487对舌鳞状细胞癌细胞细胞外信号调节激酶(ERK)的抑制作用。方法采用免疫细胞化学以及Western blot方法研究Tca8113细胞在不同浓度AG1487状态下,细胞外信号调节激酶的表达情况。结果免疫细胞化学结果显示:AG1487可明显抑制细胞核内活化ERK的表达,但对细胞质内全部ERK的表达没有明显影响;Western blot结果进一步证实,AG1487可抑制活化ERK的表达,并且,活化ERK的表达与AG1487存在剂量依赖关系。结论表皮生长因子受体抑制剂AG1487可明显抑制其MAPK信号通路,进而对细胞的分裂增殖产生抑制作用。

Objective To investigate the inhibitory effect of epidermal growth factor receptor inhibitor AG1487 on the expression of extracellular signal - regulated kinase （ERK） of Tca8113 cells. Methods Immunocytochemistry and Western blot were employed to detect the expression of active ERK and total ERK by using different concentrations of AG1487. Results The results of immunicytochemistry showed that the expression of active ERK in nucleus was inhibited by AG1487, but the expression of total ERK in cytoplasm was not affected. The results of western blot further demonstrated that AG1487 could inhibit the expression of active ERK and the inhibitor effect showed a dose- dependent pattern. Conclusion Epidermal growth factor receptor inhibitor AG1487 could inhibit the MAPK signaling pathway of Tca8113 cells. These inhibitory effects may impact the proliferation of tumor ceils.