国人肿瘤坏死因子的基因多态性与H pylori感染无相关性

No Association between polymorphism of tumor necrosis factor gene and helicobacter pylori infection in Han Chinese in central China

目的:探讨肿瘤坏死因子-α(tumor necrosis factor,TNF-α)和淋巴毒素-α(lymphotoxin-α, LT-α,即TNF-β)基因多态性与国人幽门螺杆菌(H pylori)感染的相关性,探讨宿主遗传因素对H pylori感染易感性的相关性．方法:采用序列特异性聚合酶链式反应方法分析264例武汉健康正常人外周血白细胞基因组DNA、TNF-α 308、LT-α Nco Ⅰ、LT-α AspH Ⅰ基因多态性．结果:健康正常人H pylori阳性率62．1％ (164/264),TNF-α 308、LT-α AspH Ⅰ、 LT-α Nco Ⅰ基因多态性在H pylori感染组(即 H pylori+组)和无H pylori感染组(即H pylori 组)间分布无显著性差异．中国人TNF-α 308 的基因型和等位基因频率分布与西班牙高加索人接近,但LT-α AspH Ⅰ GG型明显低于高加索人(P<0．000 1,OR=0．207,95％CI: 0．104 6-0．409 6),而CC型却显著高于对方(P =0．005 7,OR=2．043．95％CI:1．241-3．366); LT-α Nco Ⅰ GG型比高加索人明显增高(P= 0．000 4,OR=3．644．95％CI:1．677-7．915),AA 却低于高加索人(P=0．000 1,OR=0．396 3, 95％CI:0．247 1-0．635 7)．结论:中国人TNF-α 308、LT-α Nco Ⅰ、LT-α AspH Ⅰ基因多态性与H pylori的感染无相关性．

AIM： To investigate the association of gene polymorphism of tumor necrosis factor-alpha （TNF-α） 308, lymphotoxin-alpha （LT-α） with H pylori infection in Han Chinese in central China. METHODS： Specific polymerase chain reaction and restriction fragment length polymorphism （RFLP） were used to detect the genotypes of leu- kocyte genome DNA, TNF-α 308 and LT-α gene in the peripheral blood of 264 healthy volunteers from Wuhan, China. H pylori infection status was determined by a validated serological test. RESULTS： The positive rate of H pylori infection was 62.1%（164/264） in the healthy volunteers. There was no significant difference in allele frequencies of TNF-α 308, LT-α AspH Ⅰ, and LT-α NcoⅠ between H pylori positive and negative group. In comparison with Spanish Caucasian, the frequency of TNF-α 308 allele was not significantly different in Chinese, but the allele frequencies of LT-α AspH Ⅰ GG and NcoⅠ AA were markedly lower （P 〈 0.000 1, OR = 0.207, 95%CI： 0.104 6-0.409 6; P = 0.000 1, OR = 0.396 3, 95%CI： 0.247 1-0.635 7）. However, the frequencies of LT-α AspH Ⅰ CC and NcoⅠ GG were notably higher （P = 0.005 7, OR = 2.043, 95%CI： 1.241-3.366; P = 0.000 4, OR = 3.644, 95%CI： 1.677-7.915）. CONCLUSION： The polymorphisms of TNF-α 308, LT-α NcoⅠ, and LT-α AspH Ⅰ are not correlated with the susceptibility of H pylori infection in healthy Han Chinese in central China.