应用MTT吸光度法快速检测原代肺癌细胞对化疗药物的敏感性

Chemosensitivity Testing of Lung Cancer Cells Using the MTT Assay

采用改良的MTT吸光度法测试了15例肺癌标本对5种常用化疗药物的体外敏感性。结果表明,肺癌对阿霉素(ADM)、丝裂霉素C(MMC)、顺铂(DDP)的敏感率高(分别为76.9％、69.2％、53.8％);对5-氟脲嘧啶(5-FU)和长春新碱(VCR)的敏感率低(皆为23.1％)。不同类型的肺癌和同一类型的不同个体对化疗药物的敏感性差异较大。对4例标本同时进行了重复性检测。行t检验表明,同一标本做两次检测其结果无显著性差异,说明此方法重复性良好。

A modified MTT assay was adapted for chemosensitivity testing on 15 patients with lung cancer to 5 antitumor agents. The sensitivity rates of lung cancer cells exposed to adriamycin, mitomycin C and cisplatin were higher(76. 9% ,69. 2% ,53. 8% respectively) ,to 5-FU and vincristine were lower (23.1% either). The assay demonstrated a variation in drug sensitivity between not only different types of lung cancer but also different lung cancers of same type. Reproducibility testing of samples from four patients was done. There were no significant differences(.P>0. 05) between results using the same sample on two separate occasions.