间接免疫荧光法与外斐氏反应诊断恙虫病的比较

Comparison of Indirect Immunofluorescence Assay and Weil-Felix Test in Diagnosis of Tsutsugamushi Disease

采用外斐氏反应(WF-OX_K)和间接免疫荧光法(IFA),对选取的87例恙虫病患者、53名其它立克次氏体抗体(R-Ab)携带者和60名健康人检测了恙虫病抗体,并用6项指标评价所获结果。结果IFA法明显优于WF-OX_K法,对发病1周以上的患者其敏感度为96.1％,特异度为97.3％。其它几项指标也优于WF-OX_K法。作者认为:长期以来用于恙虫病血清学诊断的WF-OX_K法不应作为诊断的唯一依据,应结合临床和流行病学综合分析。而IFA法的真实性和可靠程度已基本达到辅助临床诊断的要求,也能满足流行病学调查的需要,应予以推广。

Comparison of the experimental results was reported between indirect immuofluorescence assay (IFA) and Wall-Felix test(WF-OXK)in the sanologic diagnosis among 87 cases with Tsutsugamushi disease,53 p'ersons with antibodies of Other rickettsiosis' and 60 perspns without any disease. The results weie e'valuated by 6 indicatory standards. The evaluation results showed that IFA was more cred-itable than WF-OXK,and IFA sensitivity and specificity were 96. 1% and 97. 3% respectively in the first week af the: disease. Thus it can be seen that WF-OXn method used to diagnosis the disease should be applied combining with clinical and epidemiological diagnosis. IFA method could be recom-mended because it reaches the basic standards in serological diagnosis of the clinical patients and the epidemiological investigation.