人微小病毒B19感染与Kikuchi病的相关性

Relationship between human parvovirus B19 infection and Kikuchi-Fujimoto disease

目的:研究人微小病毒B19感染与组织细胞性坏死性淋巴结炎(KFD)的相关性.方法:采用双盲法在32例KFD患者及13例正常对照淋巴结活检组织中用原位杂交和免疫组织化学染色方法检测B19病毒基因组DNA和病毒蛋白.并采用原位杂交和免疫组化双标记法确定病毒感染的细胞类型.结果:在32例KFD患者淋巴结中,B19病毒DNA和病毒蛋白阳性率分别为71.9%和59.4%,而在对照淋巴结中,二者阳性率分别为30.8%和23.1%.统计学分析显示B19病毒基因组DNA和病毒蛋白在KFD中有更高感染率(P值分别为0.011和0.027).病毒感染的细胞大部分为淋巴细胞,少数组织细胞也是病毒感染的靶细胞.结论:微小病毒B19在KFD中有更高的感染率,微小病毒B19与KFD的发病有密切关系.

AIM： To explore the relationship between parvovirus B19 infection and idiopathic Kikuchi-Fujimoto disease （KFD）. METHODS： Tissue blocks from lymph nodes of 32 idiopathic KFD patients and from normal lymph nodes of 13 normal controls were detected for parvovirus B19 DNA and viral proteins by in situ hybridization and techniques in a doubleblind manner. In situ hybridization and immunohistochemical double-staining were used to determine the type of virus-infected cells. RESULTS： In situ hybridization and immunohistochemistry revealed that the positive rates of B19 DNA and viral proteins were 71.9% and 59.4% respectively in the 32 idiopathic KFD patients. In the control group, the positive rates were 30.8% and 23.1%, respectively. Statistical analysis indicated that the positive rates of B19 DNA and proteins differed significantly between the idiopathic KFD patients and the controls （P=0.011 and P= 0.027 respectively）. The B19 virus-infected cells were mainly lymphocytes and a small number of histocytes. CONCLUSION： Lymph nodes from idiopathic KFD patients have higher parvovirus B19 infection rates than those from controls. Parvovirus B19 infection may play an important role in the pathogenesis of idiopathic KFD.