γ-干扰素对人眼组织共刺激分子及Fas/FasL分子表达的影响

Effects of γ-interferon on the expression of costimulatory molecules and Fas/FasL in human retina

目的探讨γ-干扰素对人眼组织中共刺激分子及Fas/FasL分子表达的影响。方法正常供体人眼9只用于实验。其中6只眼作视网膜平片,每只眼取12片视网膜平片;分别将视网膜平片放于24孔培养板中,共分为3组,每孔含Dulbecco改良Eagle(DMEM)/F12培养液2ml,3组γ-干扰素(IFN-γ)浓度分别为0、200、1000U/ml;37℃培养箱中培养(95%O2,5%CO2)24h后取出固定,用免疫组织化学方法检测各视网膜平片上B7-1、B7-2、Fas/FasL分子的表达。同时取另外3只正常人眼制作视网膜平片做为正常对照组,直接用免疫组织化学方法检测平片中上述分子的表达。结果正常人视网膜平片中有FasL分子的表达,但无B7-1、B7-2和Fas分子表达;当视网膜平片与IFN-γ体外培养后,视网膜中出现B7-1、B7-2和Fas分子的表达,并且FasL分子表达量增多。结论IFN-γ可通过诱导共刺激分子及Fas/FasL分子表达参与免疫反应的发生和诱导细胞凋亡,在T淋巴细胞的激活中起着重要作用。

Objective To investigate the effects of γ-interferon （IFN-γ on the expression of costimulatory molecules B7-1 （CD80） and B7-2 （CD86） and Fas/FasL in human retina. Methods Nine human eyes were obtained from the eye-bank of Zhongshan Ophthalmic Center. Six eyes were used for making retinal wholemounts, and 12 retinal wholemounts from each eye were put into the 24-hole culture board which had 2ml DMEM/F12 culture medium in each hole. The wholemounts were divided into 3 groups whose concentration of IFN-γ was 0, 200, and 1 000 U/ml respectively. After cultured in 37℃ culture box （95%O2,5%CO2） for 24 hours, the expressions of BT-1 （CD80）, B7-2 （CD86）, and Fas/FasL on these retinal wholemounts were detected by immunohistochemical method. The retinal wholemounts from 3 healthy people were detected by immunohistochemical method as the control. Results Expression of FasL but not B7-1, B7-2 or Fas was found in the control group, while the expression of B7-1, B7-2 and Fas and increased expression of FasL were found after cultured with IFN-γ. Conclusion IFN-γmay be involved in the occurrence of ocular immune response and induction of apoptosis via the stimulation of expression of costimulatory molecules and Fas/FasL, which plays an important role in the activation of T lymphocytes.