广西百色地区壮族人群白介素-1受体拮抗剂基因多态性分析

Polymorphisms of IL-1Ra Gene in Zhuang Population in Bose of Guangxi

目的探讨广西百色地区壮族人群白细胞介素-1受体拮抗剂(IL-1Ra)基因多态性分布。方法采用PCR方法对165例百色地区壮族健康人群IL-1Ra基因数目可变的串联重复(VNTR)多态性进行了观察,并结合文献进行了不同种族间的分析比较。结果百色地区壮族健康人群IL-1Ra基因型以Ⅰ/Ⅰ型(410bp)最为常见,其次为Ⅰ/Ⅱ型(410bP和240bp),Ⅰ/Ⅳ(410bp和325bP)和Ⅱ/Ⅱ型(240bp)较为少见,分布频率依次为0.855、0.127、0.006、0.012;其等位基因以Ⅰ型最为常见,其次为Ⅱ型,Ⅳ型较为少见。与美、德和非洲白人等国家人群相比,该百色地区IL-1Ra的VNTR多态性均存在显著性差异(P<0.01),与国内重庆和江苏地区人群相比无显著性差异(P>0.05)。结论IL-1Ra基因VNTR多态性在不同种族间的分布存在着明显的差异,为进一步研究IL-1Ra基因多态性与疾病的关系奠定了基础。

Objective To study the variable number of tandem repeat （VNTR） of interleukin-1 receptor antagonist （IL-1Ra） in Zhuang population in Bose and compare with different races. Methods The VNTR polymorphism of IL-1Ra was examined in 165 health adult （male 90,female 75） with polymerase chain reaction （PCR） method. Results In Zhuangpopulation of Bosein China, Ⅰ/Ⅰ （410bp） genotype was the most common, then Ⅰ/ Ⅱ （410bp and 240bp） genotype , but the Ⅰ/Ⅳ （410bp and 325bp） and Ⅱ/Ⅱ （240bp） genotypes were rare, whose frequency was 0.855, 0. 127, 0. 006 and 0.012 respectively. The genotype frequency and allele frequency of IL-1Ra （ Ⅰ / Ⅰ and Ⅰ / Ⅱ genotype） were significantly different in Bose of Guangxi in China from American, Germany and African whites populations （P 〈 0. 01）, but similar to Chongqing and Jiangsu populations in China （ P 〉 0. 05）. Moreover, we found the rare Ⅰ/Ⅳ and Ⅱ/Ⅱ genotypes in Bose of Guangxi, but not in other populations in China. Conclusion VNTR polymorphism of IL-1Ra gene is different in different ethnic populations, which may be of significance in the study of the relationship between genetics and disease association.