短暂性全脑缺血后再灌注大鼠纹皮质血管c-Fos蛋白表达及CGRP和NGF的影响被引量：8

The Effect of Calcitonin Gene Related Peptide and Nerve Growth Factor on the Expression of c-Fos in Striate Cortex Vessels of Rats with Transient Cerebral Ischemia/Reperfusion

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摘要目的研究外源性降钙素基因相关肽(CGRP)和神经生长因子(NGF)对短暂性全脑缺血后再灌注大鼠纹皮质血管c-Fos蛋白表达的影响,探讨CGRP和NGF对缺血神经元的作用机制。方法在全脑缺血后再灌注模型上,应用免疫组织化学结合显微图象分析检测了c-Fos蛋白表达。结果假手术组、缺血再灌注组、CGRP组、NGF组和CGRP+NGF组缺血后再灌注3h、1d时在纹皮质的血管及其附近未见c-Fos蛋白阳性反应。缺血后再灌注3 d时在纹皮质的血管及其附近,缺血再灌注组未见c-Fos蛋白阳性反应,但NGF组和CGRP组c-Fos蛋白阳性反应明显,合用CGRP和NGF组分别较NGF组和CGRP组c-Fos蛋白阳性反应明显增强。结论CGRP和NGF分别增强脑缺血后再灌注大鼠纹皮质血管c-Fos蛋白表达,二者联合应用强于单独应用,二者对保护缺血神经元可能有协同作用。 Objective To study the effect of calcitonin gene related peptide （CGRP） and nerve growth factor （NGF） on the expression of c-Fos protein in striate cortex vessels of rats with transient cerebral ischemia/reperfusion （I/R）. Methods Immunohistochemistry and microimage analysis were employed. Results The expression of c-Fos proteins in vessels and paravessels of striate cortex was absent in sham group, I/R group, NGF group, CGRP group and in NGF ＋ CGRP group with 3h and ld survival after I/R, and still without c-Fos expression 3 days after I/R. However, when the animals survived 3 days after I/R, the expression of c-Fos was increased markedly in vessels and paravessels of striate cortex in NGF group and CGRP group compared with/IR group, and much higher in NGF ＋ CGRP group than in NGF group and CGRP group respectively. Condusion The increased expression of c-Fos by CGRP and NGF respectively and higher increased c-Fos expression by combination of NGF and CGRP indicate that CGRP and NGF have coordinate protective effects on the ischemia neurons in brain.

作者金大成花放方秀斌

机构地区中国医科大学基础医学院解剖学教研室中国医科大学基础医学院神经生物学教研室

出处《解剖科学进展》 CAS 2006年第1期15-17,共3页 Progress of Anatomical Sciences

基金辽宁省自然科学基金(NO.619019)

关键词脑缺血 C-FOS 降钙素基因相关肽神经生长因子纹皮质大鼠 cerebral ischemia c-Fos calcitonin gene related peptide nerve growth factor striate cortex rat

分类号 R322.81 [医药卫生—人体解剖和组织胚胎学]

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参考文献10

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二级参考文献7

1Johnson FG, Bell BA, Robertson IJ, et al. Effect of calcitonin-gene-related peptide on postoperative neurological deficits after subarachnoid haemorrhage. Lancet, 1990,335(8694):869-872.
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5Holland JP, Sydserff SG, Taylor WA, et al. Calcitonin gene-related peptide reduces brain injury in a rat model of focal cerebral ischemia. Stroke, 1994,25(10):2055-2059.
6Gillardon F, Lenz C, Waschke KF, et al. Altered expression of Bcl-2, Bax, and c-Fos colocalizes with DNA fragmentation and ischemic cell damage following middle cerebral artery occlusion in rats. Mol Brain Res, 1996,40(2):254-260.
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