期刊文献+

帕金森病基因治疗的临床前和临床研究 被引量:1

Preclinical and Clinical Studies on Gene Therapy of Parkinson's Disease
下载PDF
导出
摘要 帕金森病灵长类动物模型的两种临床前基因治疗策略主要是,向脑黑质纹状体系统导入多巴胺合成酶基因或神经营养因子基因,以增加纹状体多巴胺水平或增强黑质残存神经元的存活能力。临床实验研究是将腺相关病毒介导的谷氨酸脱羧酶基因导入丘脑底核,使兴奋性神经递质谷氨酸变为抑制性神经递质GABA,从而抑制丘脑底核的靶核团苍白球内侧核和黑质网状部活性过高状态,使丘脑皮层通路的过度抑制被解除而达到治疗帕金森病的目的。 Two main strategies have being tested in pre-clinical works on gene therapy in a nonhuman primate model of Parkinson's disease, including introduction of therapeutic genes such as dopamine-synthetic enzymes or neurothophic factors in vivo or ex vivo into the nigrostriatal system to augment the striatal level of dopamine or to promote the survival of the remaining nigral neuron. In clinical trials in humans with Parkinson's disease, glutamic acid decarboxylase gene mediated by adeno-associated virus was transduced into the subthalamic nucleus where synthesis of the inhibitory neurotransmitter GABA was catalyzod by the enzyme from the excitory glutamic acid, as a result of the inhibition of overactivity of its terminal regions ( the internal segment of the globus pallidus and the substantia nigra pars reticulata), eventuaUy leading to marked attenuation of over-inhibitory thalamocortical circuit and generation of therapeutic benefit.
作者 张乐 段德义
出处 《解剖科学进展》 CAS 2006年第1期57-59,共3页 Progress of Anatomical Sciences
基金 北京市教委科技发展计划重点项目(KZ200410028011) 北京市优秀人才培养专项经费资助
  • 相关文献

参考文献11

  • 1章为,周雪.帕金森病基因治疗的研究进展[J].四川解剖学杂志,2002,10(3):167-170. 被引量:2
  • 2Mandel RJ,Rendahl KG,Snyder RO,et al.Progress in direct striatal delivery of L-DOPA via gene therapy for treatment of Parkinson's desease using recombinant adeno-associated viral vectors[J].Exp Neurol,1999,159(1):47-64.
  • 3Prasad KN,Clarkson ED,La Rosa FG,et al.Efficacy of grafted immortalized dopamine neurons in an animal model of Parkinsonism:a review[J].Mol Genet Metab,1998,65 (1):1-9.
  • 4Costantini LC,Bakowska JC,Breakefield XO,et al.Gene therapy in the CNS[J].Gene Therapy,2000,7 (2):93-109.
  • 5Howard K.First Parkinson gene therapy trial launches [ J ].Nat Biotechnol,2003,21(10):1117-1118.
  • 6During MJ,Kaplitt MG,Stern MB,et al.Subthalamic GAD gene transfer in Parkinson disease patients who are candidates for deep brain stimulation[J].Hum Gene Ther,2001,12 (12):1589-1591.
  • 7Luo J,Kaplitt MG,Fitzsimons HL,et al.Subthalamic GAD gene therapy in a Parkinson's disease rat model[J].Science,2002,298(5592):425-429.
  • 8Hopkins Tanne J.Diet supplements and gene therapy tried for Parkinson's disease[J].BMJ,2002,325(7369):851.
  • 9田季雨,陈建宗,刘澎涛,李斌.病毒载体介导的帕金森病基因治疗研究进展[J].中华老年医学杂志,2003,22(10):636-638. 被引量:2
  • 10Peck P.First Parkinson disease gene therapy trial gets off to a well-publicized and controversial start [ J ].Neurology Today,2003,3 (10):1,8,11.

二级参考文献48

  • 1Frim DM,Le HN. Gene therapy for Parkinson disease. Expert Opion Bim Ther, 2002,2 : 151-161.
  • 2Wolff J A, Fisher I J, Xu L, et al. Grafting fibroblasts genetically modified to produce L-dopa in a rat model of Parkinson disease. Proc Natl Acad Sci USA.1989.86:9011-9014.
  • 3Hudson J,Granholm AC ,Gerhardt GA, et al. Glial cell linederived neurotrophic factor augments midbrain depaminergic circuits in vivo. Brain Res Bull,36:425-432.
  • 4Cheng FC , Ni DR, Wu MC, et al. Glial cell line-derived neurotrophic factor protects against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ( MPTP)-indrs neurotoxicityin C57BL/6 mice. Neurosci Lett, 1998,25 : 87-89.
  • 5Rosenblad C,Martine ZA,Klund A, et al. Intrast viatal glial cell line-derived neurotrophic factor promotes sprouting of spared nigrostriated dopaminergic afferents and induces recovery of function in a rate model of Parkinson disease. Neuroscience. 1998. 82: 129-137.
  • 6Muramatsu S, Fujlmoto K, Ikeguehl K, et al. Behavioral recovery in a primate model of Parklnson' s disease by trip letransduction of striatal ceus with deno-associated viral vectors expressing dopamine- synthesizing enzymes. Hum Gene Ther,2002, 13: 345-354.
  • 7Fan D, Shen Y, Kang D, et al. Adeno-associated virus vectormediated triple gene transfer of dopamine synthetic enzymes. Chin Med J (Engl), 2001, 114: 1276-1279.
  • 8Mandel RJ, Snyder RO, Left SE. Recombinant Adenoassociated viral vector-mediated glial cell line-derived neurotrophic factor gene tranfer protects nigral dopamine neurons after onset of progressive degeneration in a rat model of Parkinson's Disease. Exp Neruo, 1999, 160: 201-214.
  • 9Bankeiwicz KS, Eberling JL, Kohumicka M, et al. Convection-enhanced delivery of AAV vector in Parkinsonian monkeys; In vivo detection of gene expression and restoration of dopaminergic function using pro-drug approach. Exp Neuro,2000, 164: 2-14.
  • 10Reynolds P, Dmitriev I, Curiel D. Insertion of an RGD motif into the HI loop of adenovirus fiber protein alters the destribution of transgene expression of the systemically administered vector. Gene Ther,1999, 6 : 1336-1339.

共引文献2

同被引文献24

引证文献1

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部