Interleukin-1β gene polymorphism associated with hepatocellular carcinoma in hepatitis B virus infection

AIM:To examine the effect of interleukin-l-beta (IL-1β)promoter region C-511T and IL-1 receptor antagonist(IL-1RN) polymorphism among the patients with chronichepatitis B virus (HBV) infection (HCC and non-HCC).METHODS:Genomic DNA from 136 Thai patients withchronic HBV infection (HCC=46 and non-HCC=90) and152 healthy individuals was genotyped for IL-1β genepolymorphism (-511) using polymerase chain reactionwith sequence specific primers (PCR-SSP).The variablenumber of tandem repeats (VNTR) of IL-1RN gene wasassessed by a PCR-based assay.The association betweenthese genes and status of the disease was evaluated byX^2 test.RESULTS:IL-1B-511 genotype C/C was found tobe significantly different in patients with HCC whencompared with healthy individuals (P=0.036,OR=2.29,95%CI=1.05-4.97) and patients without HCC (P=0.036,OR=2.52,95%CI=1.05-6.04).Analysis of allelefrequencies of IL-1B-511 showed that IL-1B-511 Callele was also significantly increased in patients withHCC,compared to that in healthy control (P=0.033,OR=1.72,95%CI=1.04-2.84).However,no significantassociation in IL-1RN gene was found between the twogroups.CONCLUSION:IL-1B-511C allele,which may beassociated with high IL-1B production in the liver,is agenetic marker for the development of HCC in chronic hepatitis B patients in Thai population.

AIM： To examine the effect of interleukin-l-beta （IL-113） promoter region C-511T and IL-1 receptor antagonist （IL-1RN） polymorphism among the patients with chronic hepatitis B virus （HBV） infection （HCC and non-HCC）. METHODS： Genomic DNA from 136 Thai patients with chronic HBV infection （HCC =46 and non-HCC= 90） and 152 healthy individuals was genotyped for IL-113 gene polymorphism （-511） using polymerase chain reaction with sequence specific primers （PCR-SSP）. The variable number of tandem repeats （VNTR） of IL-1RN gene was assessed by a PCR-based assay. The association between these genes and status of the disease was evaluated by X^2 test. RESULTS： IL-1B-511 genotype c/c was found to be significantly different in patients with HCC when compared with healthy individuals （P = 0.036, OR = 2.29, 95%CI = 1.05-4.97） and patients without HCC （P=0.036, OR= 2.52, 95%CI=1.05-6.04）. Analysis of allele frequencies of IL-1B-511 showed that IL-1B-511 C allele was also significantly increased in patients with HCC, compared to that in healthy control （P=0.033, OR= 1.72, 95%CI=1.04-2.84）. However, no significant association in IL-1RN gene was found between the two groups. CONCLUSION： IL-1B-511C allele, which may be associated with high IL-1B production in the liver, is a genetic marker for the development of HCC in chronic hepatitis B patients in Thai population.