VEGF in hepatocellular carcinoma and surrounding cirrhotic liver tissues

TO THE EDITORWe read with a great interest the recent work of Deli andcolleagues. in the World Journal of Gastroenterology reportingvascular endothelial growth factor (VEGF) expressionin hepatocellular carcinoma (HCC) and cirrhotic livertissues. This well-documented work shows that VEGFwas significantly higher in surrounding cirrhotic livertissues than in HCC. Authors assessed VEGF expressionusing immunohistochemistry.The

We read with a great interest the recent work of Deli and colleagues. in the World Journal of Gastroenterology reporting vascular endothelial growth factor （VEGF） expression in hepatocellular carcinoma （HCC） and cirrhotic liver tissues. This well-documented work shows that VEGF was significantly higher in surrounding cirrhotic liver tissues than in HCC. Authors assessed VEGF expression using immunohistochemistry. The immunohistochemical staining is an efficient tool to assess the percentage of cells stained positively for VEGF but is not really efficient to estimate their true VEGF content. Evaluation of the VEGF protein by an enzyme-linked immunosorbent assay 0ELISA） has been reported, by us and others, to be an efficient tool in order to assess tissue VEGF expression. We have, thus, tested whether the ELISA method might be an efficient tool in order to confirm data reporting higher amounts of VEGF in surrounding cirrhotic liver tissues than in HCC. Deli and colleagues. also correctly pointed out that basic fibroblast growth factor （bFGF） has been reported to act cooperatively on VEGF expression. We have, thus, also assessed bFGF tissue levels in order to search for a putative link between VEGF and bFGF levels in cirrhotic tissues.