PCR—SSCP法检测非小细胞肺癌中FADD基因突变的研究

PCR - SSCP Analysis of FADD Gene Mutation in Non- Small Cell Lung Cancer

目的：检测Fas相关死亡结构域蛋白（Fas—associated death domain protein，FADD）基因在人非小细胞肺癌（non—small cell lung cancer，NSCLC）中的突变情况，以探讨该基因在肺癌发生发展中的作用及机制。方法：采用聚合酶链反应-单链构象多态性分析（polymerase chain reaction and single—strand conformation polymorphism，PCR—SSCP）检测74例NSCLC原发灶癌组织及13例癌旁正常肺组织中FADD基因突变情况。结果：74例NSCLC组织中检出5例发生FADD基因突变，FADD基因突变与癌的淋巴结转移呈显著正相关（ri=0．378，P=0．001），与其它临床病理特征无关。结论：NSCLC中存在着FADD基因突变。FADD基因突变在NSCLC的发生发展中可能起着重要作用。

Objective：This report aimed at the mutation of FADD gene in human non - small cell lung cancer, and the evaluation of its possible effect and mechanism on the development of NSCLC. Methods PCR - SSCP was used to detect FADD gene mutation in 74 specimens from primary site of NSCLC tissues and 13 samples of normal adjacent lung tissues. Results： Altogether there were 5 cases of FADD gene mutation, and the analysis revealed that its mutation was positively correlated to the status of lymph node metastasis （ r, = 0. 378, P = 0. 001 ） , but no relationship was found between FADD mutation and other clinical features. Conclusion： There are FADD gene mutation in NSCLC. FADD gene mutation probably plays a crucial role in NSCLC carcinogenesis.