氯化血红素作为过氧化物模拟酶催化测定雨水中过氧化氢

Determination of Hydrogen Peroxide in Rainwater using Heroin as Mimetic Peroxidase

研究了Heroin作为过氧化物模拟酶对过氧化氢（H2O2）-4氨基安替比林（4-Aminoantipyrine，4-AAP）-对氯苯酚（P—Chlorophenol，P—CP）显色反应的催化特性及反应条件，该体系在pH9．5的条件下形成的酶催化产物在505nm处有最大的吸收，测定H2O2的表观摩尔吸光系数为2．9×10^3L·mol^-1·cm^-1。该方法简便，灵敏度和选择性较好，用于雨水中微量过氧化氢的直接测定，结果满意。

The catalytic activity and reaction conditions of Heam chloride （Hemin） on the chromogenic reaction of H2O2/4 - AAP/ p - Chlorophenol（ p - CP） has been discussed. At pH9.5 , products of this system exhibited maximum absorption at 505nm. The apparent molar absorption for determination of hydrogen peroxide was found to be 2.9 × 10^3L·mol^-1 · cm^-1 The method is simple and good selective for the direct determination of hydrogen peroxide in rainwater with satisfactory results.