复合酶法制备早籼米多孔淀粉的工艺条件

Hydrolysis Conditions for Porous Starch Preparation with Early-indica Rice by Compound Enzyming

以早籼米淀粉为原料,探讨了复合酶法制备多孔淀粉的工艺条件。在适宜条件下用一定比例的α-淀粉酶与糖化酶复合酶水解早籼米淀粉,以形成多孔淀粉。主要考查了温度、pH值、时间、酶用量和复合酶比对多孔淀粉得率、吸水率和吸附色素能力的影响。研究表明,针对多孔淀粉的得率、吸水率和对胭脂红吸附量3个指标,其最佳工艺条件不一致,以吸水率最佳为标准,最佳制备条件为温度55℃,pH4.0,时间16h,α-淀粉酶与糖化酶体积比1∶3,酶活分别为14.00KNU/g和90.00AGU/g。在此条件下经两次验证,多孔淀粉的得率分别为41.07%和43.37%,吸水率分别为148.03%和155.61%,对胭脂红的吸附量分别为9.37mg/g和9.38mg/g。

Porous starch is a good carrier as many functional components in food or medicine industry. The preparation methods for porous starch using α-amylase and glucoamylase to hydrolyze early indica rice starch were presented. Main hydro lyzing factors such as temperature, pH value, time and quantity of the enzymes were investigated by statislical method. In each case, the best technique conditions for preparing porous starch were different for three response values; yield rate, waler absorption and adsorption capacity of ponceau 4R. Based on the water absorption of porous starch, lhe best condition obtained from enzymatic experiment was lhe enzymatic temperature 55℃ ,pH 4.0 and hydrolyzing time 16 h, the ratio of α-amylase to glucoamylase in volume 1 ： 3,enzyme active 14, 00 KNU/g,90.00 AGU/g,respectively. When validated twice under the opliraised conditions, the porous starch yield rate was 41.07% and 43.37%, water absorption was 148.03% and 155.61%, adsorption capacity of ponceau 4R was 9.37 mg/g and 9.38 mg/g, respectively.