摘要
建立用HPLC-ELSD法测定蒺藜皂苷1（Terrestrosin 1）的含量。用Diamonsil C18柱；乙腈-水（36：64）为流动相。蒺藜皂苷1在15～3000弘g·ml。范围内，进样量与峰面积有良好的线性关系（n=6，r=0．9997）。回收率为98．39％，RSD为1．11％（n=9）。测定三批蒺藜皂苷1含量均〉98％。方法简便准确，可作为蒺藜皂苷1的含量测定方法。
An HPLC-ELSD method was established for determination of terrestrosin 1. A Diamonsil C18 column and evaporative light scattering detector （ELSD） were used. Acetonitrile-water （36 ： 64） were used as mobile phase. There was a good linear relationship （r=0. 99997, n= 6,）when terrestrosin 1 was 15- 3000μg·ml^-1. The recovery rate was 98.39% and RSD was 1.11% （n=9）. The content of terresrosin 1 in any of the three batches tested was greater than 98%. The method was simple and accurate. It could be used for the determination of Terrestrosin 1.
出处
《中国药事》
CAS
2006年第3期158-159,共2页
Chinese Pharmaceutical Affairs
