樟柳碱对大鼠脑缺血再灌注损伤的影响(英文)

Effect of anisodine on acute forebrain ischemia-reperfusion damage in rats

目的:研究樟柳碱对大鼠急性脑缺血及再灌注损伤的影响。方法:电灼闭塞锥动脉并夹闭颈动脉,使大鼠前脑缺血30min,放开双侧颈总动脉重灌60min,并在重灌40min时iv 2％伊文思蓝0.2mL。分别用原子吸收分光光度法,分光光度法测定前脑钙含量和伊文思蓝含量。结果:缺血重灌后,大鼠脑钙含量由对照的112±6μg/g干重脑增加至165±7μg/g干重脑,伊文思蓝含量由对照的3.3±0.3μg/g湿重脑增加至6.7±0.5μg/g湿重脑,樟柳碱,东莨菪碱可使异常增高的脑钙含量以及伊文思蓝含量明显降低。结论:樟柳碱和东莨菪碱通过降低缺血及重灌引起的脑钙积累,减轻脑损伤改善脑功能。

AIM: To study the protective effect of anisodine (Ani) on acute forebrain ischemia-reperfusion injury in rats. METHODS: Both vertebral arteries were occluded by electrocautery. Severe, but transient bilateral cerebral ischemia was produced by clamping both common carotid arteries in rats. Atomic absorption spectrophotometric and spectrophotometric methods were used to determine the contents of calcium and extravasated Evans blue (EB), respectively, remained in forebrain at 60-min recirculation after 30-min ischemia. RESULTS: At 60-min recirculation, the brain calcium contents were increased from 112 ± 6 μg/g brain dry weight in control (sham operation) group to 165 ± 7 μg/g brain dry weight with marked increase of EB extravasation. Ani (2.5 mg·kg-1, ip) , and scopolamine (Sco, 0.25 mg·kg-1, ip) decreased the elevated calcium and extravasated EB contents. CONCLUSION: Ani prevented the brain from ischemia insults through reducing intracellular calcium accumulation resulted from ischemia and reperfusion.