胆红素对腹水型肝癌细胞生长及DNA合成的光敏作用(英文)

Photosensitization of bilirubin on proliferation and DNA synthesis in ascitic hepatoma cells

目的:观察胆红素光敏反应对腹水型肝癌(Hep A)细胞DNA的影响及其机理。方法:细胞经1.0×10~5lx照光10min后加脱氧[~3H]胸苷,测DNA的合成。细胞用0.5％台盼兰染色后计数。结果:胆红素光敏反应使细胞死亡率增加;DNA合成明显受到抑制(P<0.01);且随浓度的增加和照光时间的延长而加剧。在自然光照下,照光组与避光组DNA合成没有明显区别(P>0.05)。结论:胆红素光敏反应对Hep A细胞有明显的杀伤作用;自然光照组不产生光敏反应;光敏反应的产生与~1O_2和H_2O_2密切相关,而与OH·和O_2^-无关。

AIM: To observe the effects and its mechanism of photosensitization of bilirubin on ascitic hepatoma (Hep A) cells. METHODS: After the cells were illuminated under the light (1.0 × 106 Ix) for 10 min, deoxy[3H]thymidine was added for DNA synthesis assay. The cells were dyed with 0.5 % try-pan blue and were counted. RESULTS: The cell mortality of illuminated groups were much higher lhan that of dark groups (P<0.01). The inhibitions of DNA synthesis of illuminated groups were stronger than that of dark groups ( P < 0.01). The cell mortality and inhibition of DNA synthesis of illuminated groups were positively dependent upon the concentrations of bilirubin and illuminating time. No difference of DNA synthesis between room light illumination groups and dark groups ( P < 0.05 ) . CONCLUSION: The photosensitization of bilirubin killed Hep A cells obviously. Bilirubin under room light had no effect on inhibition of DNA synthesis. The photosensitization of bilirubin was closely related to 1O2 and H2O2 and not to OH·and O2.