甲硫氨酸脑啡肽增强TNF-α产生,NK细胞活性和IL-12p35mRNA的表达(英文)

Augmentation of TNF-a production NK cell activity and IL-12 p35 mRNA expression by methionine enkephalin

目的:研究甲硫氨酸脑啡肽对机体免疫监督功能的影响。方法:测定met-enk对NK活性,抗癌细胞因子如:TNF-α和IL-12的产生和基因表达的影响。结果:Met-enk(1×10^(-8)-1×10^(-5)mol·L^(-1))能增强NK细胞活性。体外,体内均能刺激TNF-α的产生。ip 0.1及1 mg·kg^(-1)×6d可增强IL-12 p35 mRNA的表达。结论:Met-enk上调抗癌细胞因子及NK活性,在癌症监督中起一定的作用。

AIM: To study the host immune surveillance functions by the neuropeptide methionine enkephalin (met-enk) . METHODS: To measure the effects of met-enk on NK activity, the production and gene expression of anti-tumor cytokines tumor necrosis factor a (TNF-a) and interleukin-12 (IL-12) in vitro and in vivo. RESULT: Met-enk promoted NK activity at 1 × 108 - 1 × 10-5 mol·L-1, increased TNF-a production both in vitro and in vivo, and enhanced IL-12 p35 gene transcription after ip 0.1 mg·kg-1 for 6 d. CONCLUSION: The up-regulating effects of met-enk contribute to the host neuro-immunomodulating mechanism against tumors and invading antigens.