阿司匹林,硝苯地平单用和合用对大鼠肠系膜微循环的影响

Effects of aspirin and nifedipine alone or in combination on mesenteric microcirculation of rats

目的:研究阿司匹林(Asp)与硝苯地平(Nif)并用对大鼠肠系膜微循环的影响。方法:Dextran(M_r480000)360mg·kg^(-1)iv制备大鼠肠系膜急性微循环障碍(AMD)模型,采用活体微循环法观察肠系膜微循环细动,静脉血流速度(ABFV,VBFV),血流状态(BFS),血管口径(AD,VD)的变化。结果:Asp 2.5,5mg·kg^(-1),Nif0.05,0.1mg·kg^(-1),Asp+Nif(1+0.025),(2.5+0.05)mg·kg^(-1)iv可使ABFV增大各为11.1％,31.3％,18.5％,19.3％,30.5％,39.8％,VBFV则为12.5％,25.7％,12.6％,15.2％,29.6％,36.1％,BFS改善,AD增大各为4.3％,17.9％,35.9％,39.7％,15.2％,42.8％,VD则为2.2％,4.2％,26.2％,27.4％,3.4％,28.9％,CN增多,ivAsp+Nif可明显逆转AMD。结论:Asp增快ABFV,VBFV作用优于Nif,而Nif扩AD,VD作用强于Asp,两者合用呈协同效应,并对dextran诱导的AMD具有拮抗作用。

AIM: To study the effects of the combination of aspirin (Asp) and nifedipine (Nif) on mesenteric mi-crocirculation of rats. METHODS' Acute microcir-culation disturbance (AMD) was produced by high molecular weight dextran (Mr 480 000, 360 mg ·kg-1 iv) . Arteriole and venule blood flow velocity and diameter (ABFV, VBFV, AD, VD) and blood flow state (BFS) were observed by intravital micro-circulation method. RESULTS: Asp 2.5, 5 mg ·kg-1, Nif 0.05, 0.1 mg·kg-1, Asp + Nif (1 + 0.025), (2.5 + 0.05) mg·kg-1, iv had the significant increase of 11.1 %, 31.3 %, 18.5 %, 19.3 %, 30.5 %, 39.8 % of ABFV and 12.5 %, 25.7 %, 12.6 %, 15.2 %, 29.6 %, 36.1 % of VBFV respectively, the marked improvement of BFS, and the distinctive increase of 4.3 %, 17.9 %, 35.9 %, 39.7 %, 15.2 %, 42.8 % of AD and 2.2 %, 4.2 %, 26.2 %, 27.4 %, 3.4 %, 28.9 % of VD separately, and got a raise in the number of capillaries. Asp + Nif ( 1 + 0.025), 2.5 + 0.05) mg·kg-1 iv could reverse AMD. CONCLUSION: Asp was superior to Nif in the increase of BFV, but Nif was superior to Asp in expansion of blood vessel. Asp in combination with Nif produced marked synergistic action and protection againist AMD.