苍耳愈伤组织诱导及继代培养研究

Studies on callus induction and subculture of Xanthium sibiricum

以苍耳叶片、叶柄及茎段为外植体，在MS附加不同种类和浓度激素配比的培养基上，对苍耳愈伤组织进行诱导培养，并对生长迅速、性状好的愈伤组织进行了研究．结果表明：不同激素配比和不同培养方式对愈伤组织诱导率、生长量和性状有明显的影响．在光照培养下，当MS＋6-BA2mg／L＋NAA0．5～1mg／L时愈伤组织诱导率高且生长好．

The leaf blade,leaf stalk and stem of Xanthium sibiricum were served as explants, MS medium added different kinds of hormone and proportions was used for indueing and eulturing the callus of Xanthium sibiricum. At the same time,how to get eallus whieh had rapid growth and good properties was studied. The results showed that different proportion of hormones and different ways of culture had strong influenee on the induction rate, growing amount and properties of the eallus. The growth of callus was rapid and induetion rate was the highest with 6-BA 2 mg/L ＋NAA 0.5-1 mg/L and light eulture.