甘薯的组织培养

Tissue culture of Ipomoea batatas

以甘薯的叶片为外植体进行组织培养,结果表明:适合甘薯愈伤组织诱导的培养基是MS+KT 2 mg/L+2,4-D 0.5 mg/L和MS+KT 2 mg/L+NAA 0.5 mg/L,适宜愈伤组织分化出根的分化培养基是MS+KT 0.5 mg/L或MS+KT 2 mg/L+NAA 0.5mg/L或MS+KT 2 mg/L+2,4-D 0.5 mg/L,适宜根分化出试管苗的分化培养基为MS+KT 2 mg/L+NAA 0.5 mg/L;增殖培养基为MS;生根的培养基为1/2 MS.

The leaf blade of Ipomoea batatas were used as explants for tissue culture. The results showed ：The optimum medium for callus induction was MS＋KT 2 mg/L＋2,4-D 0.5 mg/L and MS＋KT 2 mg/L＋NAA 0.5 rag/L, mediums MS＋KT 0. 5 mg/L,MS＋KT 2 mg/L＋NAA 0.5 mg/L or MS＋KT 2 mg/L＋2,4-D 0.5 mg/L were suitable for rooting,MS＋KT 2 mg/L＋NAA 0.5 mg/L was the best for seedling production,MS for subculture and 1/2 MS for rooting.