摘要
以甘薯的叶片为外植体进行组织培养,结果表明:适合甘薯愈伤组织诱导的培养基是MS+KT 2 mg/L+2,4-D 0.5 mg/L和MS+KT 2 mg/L+NAA 0.5 mg/L,适宜愈伤组织分化出根的分化培养基是MS+KT 0.5 mg/L或MS+KT 2 mg/L+NAA 0.5mg/L或MS+KT 2 mg/L+2,4-D 0.5 mg/L,适宜根分化出试管苗的分化培养基为MS+KT 2 mg/L+NAA 0.5 mg/L;增殖培养基为MS;生根的培养基为1/2 MS.
The leaf blade of Ipomoea batatas were used as explants for tissue culture. The results showed :The optimum medium for callus induction was MS+KT 2 mg/L+2,4-D 0.5 mg/L and MS+KT 2 mg/L+NAA 0.5 rag/L, mediums MS+KT 0. 5 mg/L,MS+KT 2 mg/L+NAA 0.5 mg/L or MS+KT 2 mg/L+2,4-D 0.5 mg/L were suitable for rooting,MS+KT 2 mg/L+NAA 0.5 mg/L was the best for seedling production,MS for subculture and 1/2 MS for rooting.
出处
《甘肃农业大学学报》
CAS
CSCD
2006年第1期113-115,共3页
Journal of Gansu Agricultural University
关键词
甘薯
组织培养
愈伤组织
Ipomoea batatas
tissue culture
callus