葛根素对慢性低O_2高CO_2大鼠学习记忆的影响

Effect of puerarin on learning and memory ability in rats with chronic hypoxic hypercapnia

目的:探讨不同剂量葛根素对低O2高CO2大鼠学习记忆能力和脑内胆碱乙酰转移酶(cholineacetyl-transferase,ChAT)活性的影响。方法:建立慢性低O2高CO2大鼠模型,并给予大(50mg/kg)、小(25mg/kg)剂量葛根素干预。用Morris水迷宫检测学习记忆能力,用放射化学法测定大鼠皮层、海马ChAT活性。结果:慢性低O2高CO2处理后,与对照组相比,模型组大鼠平均潜伏期明显延长(P<0.01),各脑区ChAT活性均显著降低(P<0.01)。药物干预后,与模型组相比,大、小剂量组大鼠平均潜伏期缩短(P<0.05),各脑区ChAT活性均显著升高(P<0.05),而大小剂量组间差异无显著性(P>0.05)。结论:葛根素可改善慢性低O2高CO2大鼠学习记忆能力并提高其脑内ChAT活性。

Objective To investigate the effect of puerarin on learning-memory ability and choline acetyl-transferase （CHAT） activity in the brain of rats with chronic hypoxic hypercapnia. Methods After the construction of the rat model of chronic hypoxic hypereapnia, puerarin （30 mg/kg, ip or 50 mg/kg, ip） was administered to the hypoxic hypercapnic rats. Their learning-memory ability was evaluated by using Morris water maze and their ChAT activity in two different brain regions （cerebral cortexand hippocampus） was determined by radiochemical method. Results The performance of Morris water maze test for hypoxic hypercapnic rats was worse than that of the test for the rats free of hypoxic hypercapnia （ P 〈 0.01 ） and ChAT activity in the two brain regions in rats with chronic hypoxic hypercapnia decreased significantly （ P 〈 0. 01 ）. Compared with that of the test for hypoxic hypercapnic rats without puerarin, the performance of the test for the rats in the high-dose and low-dose experimental groups was improved （ P 〈 0.05） and ChAT activity in the two brain regions in rats with puerafin increased significantly （ P 〈 0.05 ）, but there is no significant difference between these two experimental groups. Conclusion Puerarin can improve learning-memory ability and increase ChAT activity in the brain of rats with chronic hypoxic hypercapnia.