前S1蛋白与HBV-DNA检测在乙肝病毒复制时的临床价值

Clinical Signification of Detecting Pre-S1 Protein and HBV-DNA for Identifying Viral Replication in Patients with HBV

目的通过对前S1蛋白(Pre-S1蛋白)和HBV-DNA检测的比较,评价在HBeAg阴性乙肝患者中,前S1蛋白在诊断乙肝病毒复制的临床价值。方法收集本院门诊乙型病毒性肝炎患者血清标本52例,均经临床确诊为乙肝感染者。用酶联吸附测试法(ELISA)检测血清中HBeAg、Pre-S1蛋白,用多聚酶链反应(PCR)检测HBV-DNA。结果以乙肝病毒HBV-DNA≥103拷贝/mL为判断乙肝病毒存在复制的标准,经检验,HBV-DNA与Pre-S1蛋白的阳性率无统计学差异(P=0.056),而HBV-DNA与HBeAg的阳性率有统计学差异(P=0.002)。25名HBeAg阴性患者中,15名患者HBV-DNA阳性,13名患者Pre-S1蛋白阳性。Pre-S1蛋白与HBV-DNA的表达几乎是一致的,无统计学差异。结论Pre-S1蛋白是诊断乙肝病毒复制的非常有临床价值的血清学指标。

Objective To evaluate clinical signification of Pre-S1 Protein in patients with HBV without HBeAg through comparing Pre-S1 Protein and HBV-DNA. Methods Sera samples were collected from 52 outpatients with HBV confirmed by clinical methods. HBeAg, Pre-S1 Protein were detected with ELISA; HBV-DNA is detected with PCR. Results Positive rates of expressing HBV-DNA （≥ 10^3 copies/mL） and Pre-S1 Protein are similar. There are no significant difference between them by X^2 test （P = 0.056） . But there are significant difference between HBV-DNA and HBeAg （P = 0.002） . The HBV-DNA-positives are 15 of 25 HBeAg-negative in 52 patients with HBV, in which 13 are Pre-S1 Protein -positive. Conclusion Pre-S1 Protein is a valuable serological marker for HBV replication without HBeAg.