染色体8p上FGL1基因可能与肝癌转移相关

PGL1,a Candidate Metastatic Suppressor Gene of Human Hepatocellular Carcinoma

目的:比较不同转移潜能的肝癌组织的基因表达谱差异,结合以往研究发现的有关肝癌转移相关分子遗传学异常, 以发现与肝癌转移相关基因。方法:应用基因芯片技术,比较不同转移潜能的肝癌组织标本的基因表达谱,并应用RT- PCR方法对差异表达较明显的基因进行了验证。姑果:不伴转移与伴有转移的肝癌组织之间,有989个基因存在差异表达,其中10个位于染色体8p上。选取定位于8p21．3-p22的类纤维蛋白原1基因(FGL1)(fibrinogen-like 1,同义于 HFREP1,LFIRE1),用RT-PCR的方法进行验证,结果证明FGL1在伴有转移的肝癌组织中表达明显下调。结论:不同转移潜能的肝癌组织中基因表达谱存在明显差别。染色体8p上可能存在抑制肝癌转移的基因。FGL1基因可能是一候选肝癌转移抑制基因,值得进一步研究。

To screen and identify the genes relating to the metastasis of human hepatocellular carcinoma （HCC）. Methods：The difference of gene expression profiles between the HCC tissue with and without metastasis were analyzed by oligo nucleotide microarray. Based on the findings of this gene expression profiling and the previous molecular cytogenetic studies, the leading differently expressed genes were identified and selected for further analysis by semi-quantitative RT-PCR Results： 989 differently expressed genes were detected between the HCC tissues with and without metastasis. One of the leading candidate genes on chromosome 8p, FGL1, was identified. And RT-PCR analysis demonstrated that FGL1 gene was significantly down-regulated in the HCC tissues with metastasis, which further confirmed the results of the gene chips technique. Conclusion：The gene expression profiles were significantly different between the HCC tissues with and without metastasis. FGL1 on chromosome 8p might possess metastasis suppressor activity in HCC.