兔连续性神经瘤模型的建立及其应用评价

Constructing an Experimental Model of the Neuroma-in-continuity in Rabbits and Evaluating Its Applicatioa

目的:鉴于目前尚缺少连续性神经瘤损伤的哺乳类动物模型,本实验通过造成兔腓神经部分损伤,形成连续性神经瘤,为进一步研究提供基础。方法:16只兔子一侧腓神经的部分束被切除,6用后损伤段神经组织切片经HE、luxol fast blue,以及Van-Gieson组化染色证实形成典型神经瘤病理改变;以建立损伤模型的8只兔子健侧作为对照,行电生理检查运动神经传导速度(MCV)、复合肌肉动作电位(CMAP)。结果:腓神经形成连续性神经瘤后电生理改变为:MCV减慢(P <0．05),CAMP波幅降低(P<0．05)。结论:腓神经的部分损伤方法可以有效地建立连续性神经瘤型神经损伤的模型,该模型稳定、实用,可用于进一步探讨连续性神经瘤治疗相关的研究。

Objective： In view of being short of the mammalian model in neuroma-in-continuity, the experiment injured the part of peroneal nerve to the formation of the neuroma-incontinuity and was applied to the foundation of farther research. Methods：One lateral peroneal nerves of sixteen New Zeland rabbits（male,weighting 2. 25 kg±0. 25 kg） were resected the damaged nervous tissues＇ slices were shown to the typical pathological changes of neuroma by the stain of HE,luxol fast blue and Van-Gieson after six weeks. As compared with the healthy sides of eight model rabbits, motor nerve conduction velocity（MCV） ,compound motor action potential（CMAP） were performed at the sixth weeks. Results： The injured nerve formed the typical pathological changes of neuroma after six weeks. MCV in the neuroma＇ sides was significantly slowly than contralateral sides（32. 74 ± 7. 54 m/s vs 55. 77 ± 9. 35 m/s P d0. 01 ） ,CMAP was lower than healty sides （6. 95 ± 2. 48 mv vs 19. 40 ±6. 89 my P〈0. 01 ）. Conclusion：The method of partly injuring the peroneal nerve can effectively set up the model of the neuroma-in-continuity, and it is an ideal,stable and practical model to investigate the effect of the treatment in neuroma-in-continuity.